The transcription factor OsMYBc and an E3 ligase regulate expression of a K+ transporter during salt stress
| العنوان: | The transcription factor OsMYBc and an E3 ligase regulate expression of a K+ transporter during salt stress |
|---|---|
| المؤلفون: | Longyun Xiao, Yiyuan Shi, Rong Wang, Yu Feng, Lesheng Wang, Hongsheng Zhang, Xingyu Shi, Guangqin Jing, Ping Deng, Tengzhao Song, Wen Jing, Wenhua Zhang |
| المصدر: | Plant Physiol |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Symporters, Physiology, Ubiquitin-Protein Ligases, Sodium, Oryza, Focus Issue on Evolution of Plant Structure and Function, Plant Science, Plants, Genetically Modified, Salt Stress, Gene Expression Regulation, Plant, Potassium, Genetics, Cation Transport Proteins, Plant Proteins, Transcription Factors |
| الوصف: | Sodium (Na+) and potassium (K+) homeostasis is essential for plant survival in saline soils. A member of the High-Affinity K+ Transporter (HKT) family in rice (Oryza sativa), OsHKT1;1, is a vital regulator of Na+ exclusion from shoots and is bound by a MYB transcription factor (OsMYBc). Here, we generated transgenic rice lines in the oshkt1;1 mutant background for genetic complementation using genomic OsHKT1;1 containing a native (Com) or mutated (mCom) promoter that cannot be bound by OsMYBc. In contrast to wild-type (WT) or Com lines, the mCom lines were not able to recover the salt-sensitive phenotype of oshkt1;1. The OsMYBc-overexpressing plants were more tolerant to salt stress than WT plants. A yeast two-hybrid screen using the OsMYBc N-terminus as bait identified a rice MYBc stress-related RING finger protein (OsMSRFP). OsMSRFP is an active E3 ligase that ubiquitinated OsMYBc in vitro and mediated 26S proteasome-mediated degradation of OsMYBc under semi-in vitro and in vivo conditions. OsMSRFP attenuated OsMYBc-mediated OsHKT1;1 expression, and knockout of OsMSRFP led to rice salt tolerance. These findings uncover a regulatory mechanism of salt response that fine-tunes OsHKT1;1 transcription by ubiquitination of OsMYBc. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d78dc6e5569807b41a56ea669ef7fd81 https://doi.org/10.1093/plphys/kiac283 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d78dc6e5569807b41a56ea669ef7fd81 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
|---|