A Nude Mouse Model of Orthotopic Liver Transplantation of Human Hepatocellular Carcinoma HCCLM3 Cell Xenografts and the Use of Imaging to Evaluate Tumor Progression
| العنوان: | A Nude Mouse Model of Orthotopic Liver Transplantation of Human Hepatocellular Carcinoma HCCLM3 Cell Xenografts and the Use of Imaging to Evaluate Tumor Progression |
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| المؤلفون: | Tiantian Fu, Zhiting Xu, Wen-Ping Wang, Hong Ding, Yu-li Zhu |
| المصدر: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| بيانات النشر: | International Scientific Literature, Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | CD31, Male, Vascular Endothelial Growth Factor A, Pathology, medicine.medical_specialty, Carcinoma, Hepatocellular, Transplantation, Heterologous, CD34, Mice, Nude, Antigens, CD34, 030204 cardiovascular system & hematology, 03 medical and health sciences, chemistry.chemical_compound, Mice, 0302 clinical medicine, Nude mouse, Cell Line, Tumor, medicine, Carcinoma, Animals, Humans, Cell Proliferation, Mice, Inbred BALB C, biology, business.industry, Animal Study, Liver Neoplasms, General Medicine, biology.organism_classification, medicine.disease, Actins, Liver Transplantation, Transplantation, Vascular endothelial growth factor, Platelet Endothelial Cell Adhesion Molecule-1, Disease Models, Animal, chemistry, Liver, Tumor progression, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, Models, Animal, Heterografts, business, Carcinoma in Situ |
| الوصف: | BACKGROUND This study aimed to develop a nude mouse model of orthotopic liver transplantation of HCCLM3 human hepatocellular carcinoma (HCC) cell xenografts and the use of imaging and histology to evaluate tumor development and progression. MATERIAL AND METHODS HCCLM3 cells were injected subcutaneously into 25 healthy male athymic BALB/c (nu/nu) nude mice. The tumors that developed were transplanted into the liver of a new set of nude mice. After four weeks and six weeks, the mice were imaged using ultrasound (US), software-assisted contrast-enhanced ultrasound (CEUS), fluorodeoxyglucose-positron emission tomography (FDG-PET). Histology was performed on the liver and liver tumors, and included immunohistochemistry for vascular endothelial growth factor (VEGF), CD31, CD34, and alpha-smooth muscle actin (alpha-SMA). RESULTS The success rate for orthotopic tumor transplantation in the mouse liver was 90% (18/20). Liver tumors measured 11.8±2.6 mm in diameter and 525.9±250.8 mm3 in volume on the sixth week. CEUS showed rapid wash-in and washout in the liver tumors, and PET showed low tumor cell metabolism. Bone metastases were present in 45% (9/20) of mice in the sixth week. Immunohistochemistry showed positive expression for VEGF, CD31, CD34, and alpha-SMA. CONCLUSIONS The nude mouse orthotopic liver transplantation model of human HCC was shown to be a reliable model that has the potential for future research on the pathogenesis and progression of HCC and studies on drug development. |
| اللغة: | English |
| تدمد: | 1643-3750 1234-1010 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7e41dd21f72cf4e8e3809f857af183e http://europepmc.org/articles/PMC6880650 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d7e41dd21f72cf4e8e3809f857af183e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16433750 12341010 |
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