The maternal to zygotic transition regulates genome-wide heterochromatin establishment in the zebrafish embryo
| العنوان: | The maternal to zygotic transition regulates genome-wide heterochromatin establishment in the zebrafish embryo |
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| المؤلفون: | Mary G. Goll, Kathrin Laue, Srivarsha Rajshekar, Abigail J. Courtney, Zachary A. Lewis |
| المصدر: | Nature Communications Nature Communications, Vol 10, Iss 1, Pp 1-10 (2019) |
| بيانات النشر: | Nature Publishing Group UK, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, animal structures, Embryo, Nonmammalian, Euchromatin, Transcription, Genetic, Heterochromatin, Science, General Physics and Astronomy, Embryonic Development, 02 engineering and technology, Biology, General Biochemistry, Genetics and Molecular Biology, Chromatin remodeling, Article, 03 medical and health sciences, Histone H3, Transcription (biology), Animals, lcsh:Science, Zebrafish, Multidisciplinary, fungi, Gene Expression Regulation, Developmental, General Chemistry, Zebrafish Proteins, 021001 nanoscience & nanotechnology, biology.organism_classification, Chromatin, Cell biology, MicroRNAs, 030104 developmental biology, embryonic structures, Maternal to zygotic transition, lcsh:Q, 0210 nano-technology |
| الوصف: | The segregation of eukaryotic genomes into euchromatin and heterochromatin represents a fundamental and poorly understood process. Here, we demonstrate that genome-wide establishment of heterochromatin is triggered by the maternal to zygotic transition (MZT) during zebrafish embryogenesis. We find that prior to MZT, zebrafish lack hallmarks of heterochromatin including histone H3 lysine 9 trimethylation (H3K9me3) and condensed chromatin ultrastructure. Global establishment of heterochromatic features occurs following MZT and requires both activation of the zygotic genome and degradation of maternally deposited RNA. Mechanistically, we demonstrate that zygotic transcription of the micro RNA miR-430 promotes degradation of maternal RNA encoding the chromatin remodeling protein Smarca2, and that clearance of Smarca2 is required for global heterochromatin establishment in the early embryo. Our results identify MZT as a key developmental regulator of heterochromatin establishment during vertebrate embryogenesis and uncover functions for Smarca2 in protecting the embryonic genome against heterochromatinization. Eukaryotic genomes are segregated into euchromatin and heterochromatin. Here the authors show that heterochromatin establishment during zebrafish embryo development is controlled by zygotic transcription of miR-430 and subsequent degradation of maternal transcripts encoding the chromatin remodeling protein Smarca2. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7e439a8d6cb31049263a242fa7da873 http://europepmc.org/articles/PMC6449393 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d7e439a8d6cb31049263a242fa7da873 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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