Dynamic organization of cortical actin filaments during the ooplasmic segregation of ascidianCionaeggs
| العنوان: | Dynamic organization of cortical actin filaments during the ooplasmic segregation of ascidianCionaeggs |
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| المؤلفون: | Hirokazu Ishii, Tomomi Tani |
| المصدر: | Molecular Biology of the Cell |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, Cytoplasm, Cell division, Zygote, Polarity in embryogenesis, 01 natural sciences, 03 medical and health sciences, Cell cortex, Animals, Urochordata, Molecular Biology, Actin, 030304 developmental biology, 0303 health sciences, biology, Articles, Cell Biology, biology.organism_classification, Actins, Cell biology, Ciona, Actin Cytoskeleton, Germ Cells, Fertilization, Ran, Oocytes, Female, 010606 plant biology & botany |
| الوصف: | Spatial reorganization of cytoplasm in zygotic cells is critically important for establishing the body plans of many animal species. In ascidian zygotes, maternal determinants (mRNAs) are first transported to the vegetal pole a few minutes after fertilization and then to the future posterior side of the zygotes in a later phase of cytoplasmic reorganization, before the first cell division. Here, by using a novel fluorescence polarization microscope that reports the position and the orientation of fluorescently labeled proteins in living cells, we mapped the local alignments and the time-dependent changes of cortical actin networks in Ciona eggs. The initial cytoplasmic reorganization started with the contraction of vegetal hemisphere approximately 20 s after the fertilization-induced [Ca2+] increase. Timing of the vegetal contraction was consistent with the emergence of highly aligned actin filaments at the cell cortex of the vegetal hemisphere, which ran perpendicular to the animal–vegetal axis. We propose that the cytoplasmic reorganization is initiated by the local contraction of laterally aligned cortical actomyosin in the vegetal hemisphere, which in turn generates the directional movement of cytoplasm within the whole egg. |
| تدمد: | 1939-4586 1059-1524 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8e9f569603aec1af1913d84f8a07ab8 https://doi.org/10.1091/mbc.e20-01-0083 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d8e9f569603aec1af1913d84f8a07ab8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
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