N-acetylcysteine and taurine prevent hyperglycemia-induced insulin resistance in vivo: possible role of oxidative stress
| العنوان: | N-acetylcysteine and taurine prevent hyperglycemia-induced insulin resistance in vivo: possible role of oxidative stress |
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| المؤلفون: | I. George Fantus, Adria Giacca, Neehar Gupta, Tony K.T. Lam, C. Andrew Haber, Elena Bogdanovic, Zhiwen Yu, Tracy Goh |
| المصدر: | American Journal of Physiology-Endocrinology and Metabolism. 285:E744-E753 |
| بيانات النشر: | American Physiological Society, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Blood Glucose, Male, medicine.medical_specialty, Taurine, Physiology, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Glucose uptake, Biology, medicine.disease_cause, Rats, Sprague-Dawley, Lipid peroxidation, chemistry.chemical_compound, Insulin resistance, Physiology (medical), Internal medicine, medicine, Animals, Insulin, Muscle, Skeletal, chemistry.chemical_classification, Reactive oxygen species, medicine.disease, Malondialdehyde, Acetylcysteine, Rats, Oxidative Stress, Glucose, Endocrinology, chemistry, Insulin Resistance, Oxidative stress |
| الوصف: | Exposure to high concentrations of glucose and insulin results in insulin resistance of metabolic target tissues, a characteristic feature of type 2 diabetes. High glucose has also been associated with oxidative stress, and increased levels of reactive oxygen species have been proposed to cause insulin resistance. To determine whether oxidative stress contributes to insulin resistance induced by hyperglycemia in vivo, nondiabetic rats were infused with glucose for 6 h to maintain a circulating glucose concentration of 15 mM with and without coinfusion of the antioxidant N-acetylcysteine (NAC), followed by a 2-h hyperinsulinemic-euglycemic clamp. High glucose (HG) induced a significant decrease in insulin-stimulated glucose uptake [tracer-determined disappearance rate (Rd), control 41.2 ± 1.7 vs. HG 32.4 ± 1.9 mg · kg–1· min–1, P < 0.05], which was prevented by NAC (HG + NAC 45.9 ± 3.5 mg · kg–1· min–1). Similar results were obtained with the antioxidant taurine. Neither NAC nor taurine alone altered Rd. HG caused a significant (5-fold) increase in soleus muscle protein carbonyl content, a marker of oxidative stress that was blocked by NAC, as well as elevated levels of malondialdehyde and 4-hydroxynonenal, markers of lipid peroxidation, which were reduced by taurine. In contrast to findings after long-term hyperglycemia, there was no membrane translocation of novel isoforms of protein kinase C in skeletal muscle after 6 h. These data support the concept that oxidative stress contributes to the pathogenesis of hyperglycemia-induced insulin resistance. |
| تدمد: | 1522-1555 0193-1849 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8fd7ffcc5cdadbbc691e2fbda7c1e5f https://doi.org/10.1152/ajpendo.00355.2002 |
| رقم الأكسشن: | edsair.doi.dedup.....d8fd7ffcc5cdadbbc691e2fbda7c1e5f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221555 01931849 |
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