Good's buffers have various affinities to gold nanoparticles regulating fluorescent and colorimetric DNA sensing
| العنوان: | Good's buffers have various affinities to gold nanoparticles regulating fluorescent and colorimetric DNA sensing |
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| المؤلفون: | Woohyun J. Moon, Miao Li, Kellie Chong, Jeffy Yang, Fang Zhang, Po-Jung Jimmy Huang, Guomei Zhang, Juewen Liu, Qianyi Ma |
| المصدر: | Chemical Science |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | HEPES, Inorganic chemistry, 02 engineering and technology, General Chemistry, Surface-enhanced Raman spectroscopy, 010402 general chemistry, 021001 nanoscience & nanotechnology, Phosphate, 01 natural sciences, Buffer (optical fiber), 0104 chemical sciences, MOPS, chemistry.chemical_compound, Chemistry, Adsorption, chemistry, Colloidal gold, Good's buffers, 0210 nano-technology |
| الوصف: | Citrate-capped gold nanoparticles (AuNPs) are highly important for sensing, drug delivery, and materials design. Many of their reactions take place in various buffers such as phosphate and Good's buffers. The effect of buffer on the surface properties of AuNPs is critical, yet this topic has not been systematically explored. Herein, we used halides such as fluoride, chloride, and bromide as probes to measure the relative adsorption strength of six common buffers. Among them, HEPES had the highest adsorption affinity, while MES, citrate and phosphate were weakly adsorbed with an overall ranking of HEPES > PIPES > MOPS > MES > citrate, phosphate. The adsorption strength was reflected from the inhibited adsorption of DNA and from the displacement of pre-adsorbed DNA. This conclusion is also supported by surface enhanced Raman spectroscopy. Furthermore, some buffer molecules did not get adsorbed instantaneously, and the MOPS buffer took up to 1 h to reach equilibrium. Finally, a classic label-free AuNP-based colorimetric sensor was tested. Its sensitivity increased by 15.7-fold when performed in a MES buffer compared to a HEPES buffer. This study has articulated the importance of buffer for AuNP-based studies and how it can improve sensors and yield more reproducible experimental systems. Aside from maintaining pH, Good's buffers can be adsorbed on gold nanoparticles with different affinities, affecting the stability and its fluorescent and colorimetric sensing performance. |
| تدمد: | 2041-6520 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9b1cb0582804dfe30662b86e3ee35f5 https://pubmed.ncbi.nlm.nih.gov/34094129 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d9b1cb0582804dfe30662b86e3ee35f5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20416520 |
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