Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes
| العنوان: | Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes |
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| المؤلفون: | Yang Wang, Zhiqiang Cheng, Nan Lou, Hua‑Jun Sun, Rong‑Gui Li, Xiaomei Wang |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | Spandidos Publications, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, proliferation, Kruppel-Like Transcription Factors, Melanoma, Experimental, Apoptosis, Biology, Transfection, Biochemistry, Proto-Oncogene Proteins c-myc, Kruppel-Like Factor 4, Mice, 03 medical and health sciences, 0302 clinical medicine, SOX2, Cell Line, Tumor, reprogramming factors, melanoma, Genetics, Animals, Cyclin B1, Promoter Regions, Genetic, Molecular Biology, Cell Proliferation, bcl-2-Associated X Protein, Cell growth, SOXB1 Transcription Factors, Articles, Janus Kinase 2, Cell cycle, Cellular Reprogramming, Gene Expression Regulation, Neoplastic, 030104 developmental biology, Proto-Oncogene Proteins c-bcl-2, Oncology, Cell culture, KLF4, Doxycycline, 030220 oncology & carcinogenesis, gene expression, Neoplastic Stem Cells, Cancer research, Molecular Medicine, Stem cell, Octamer Transcription Factor-3, Reprogramming, Plasmids |
| الوصف: | Uncontrolled proliferation and defective apoptosis are two major factors responsible for maintaining the malignant properties of melanoma cells. Our previous study demonstrated that induced expression of four reprogramming factors remodeled the phenotype of B16‑F10 mouse melanoma cells into melanoma stem cells. The present study was conducted to investigate the effect of the four Yamanaka reprogramming factors, namely Oct4, Sox2, Klf4 and c‑Myc (OSKM), on the proliferation and apoptosis of melanoma cells, and to identify the responsible molecular signals. The results identified that expression of the four reprogramming factors was highly induced by doxycycline treatment in the stable melanoma cell clone that was transfected with a plasmid expressing these factors, driven by the Tet‑On element. It was further confirmed that induced expression of these factors enhanced the proliferation and suppressed the apoptosis of the melanoma cells. In addition, induced OSKM expression increased cell proliferation, accelerated the progression of the cell cycle, and upregulated the mRNA expression levels of Janus kinase 2 (JAK2) and Cyclin‑B1. Induced expression of these factors also decreased the apoptosis, as well as upregulated B‑cell lymphoma 2 (BCL‑2) and downregulated BCL‑2‑associated X (BAX) mRNA expression levels. Taken together, the results suggested that upregulated JAK2 and Cyclin‑B1 may be responsible for the enhanced proliferation of melanoma cells, and that BCL‑2 upregulation and BAX downregulation may account for the suppressed apoptosis of these cells. |
| تدمد: | 1791-3004 1791-2997 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da852748515c4dff952bfde6967f75e3 https://doi.org/10.3892/mmr.2018.9753 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....da852748515c4dff952bfde6967f75e3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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