Antifungal activity and mechanism of action of Ou-gon (Scutellaria root extract) components against pathogenic fungi
| العنوان: | Antifungal activity and mechanism of action of Ou-gon (Scutellaria root extract) components against pathogenic fungi |
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| المؤلفون: | Osamu Yamamoto, Yayoi Nishiyama, Eishin Morita, Duerna Tie, Kyaw Zaw Hein, Xia Da |
| المصدر: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Spectrometry, Mass, Electrospray Ionization, Antifungal Agents, Scutellaria, lcsh:Medicine, Trichophyton rubrum, Plant Roots, Article, Aspergillus fumigatus, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Wogonin, Trichophyton, Candida albicans, Mode of action, lcsh:Science, Membrane Potential, Mitochondrial, Multidisciplinary, biology, Plant Extracts, lcsh:R, Fungi, biology.organism_classification, Baicalein, 030104 developmental biology, chemistry, Biochemistry, Flavanones, Scutellaria baicalensis, lcsh:Q, Reactive Oxygen Species, 030217 neurology & neurosurgery, Chromatography, Liquid |
| الوصف: | Ou-gon, an extract from Scutellaria baicalensis Georgi root, has been shown to exhibit pronounced antifungal activity. The present study aimed to identify antifungal components of Ou-gon and to determine their mechanism of action against pathogenic fungi. Antifungal activity was assessed by the microbroth dilution method using four common human pathogenic fungi, Trichophyton rubrum, Trichophyton mentagrophytes, Aspergillus fumigatus, and Candida albicans. Components of crude Ou-gon extract were separated by reversed-phase high-performance liquid chromatography. Active antifungal components were identified by liquid chromatography-electrospray ionization tandem mass spectrometry. Terminal deoxynucleotidyl transferase dUTP nick end-labelling assay, SYTOX® green uptake assay, determination of intracellular reactive oxygen species and mitochondrial membrane potential as well as microscopy (confocal laser microscopy, scanning and transmission electron microscopy) were used to probe the mode of action. Two components with potent antifungal activity, baicalein and wogonin, were identified in Ou-gon. Baicalein showed potent antifungal activity against the four fungi tested. Wogonin displayed antifungal activity against all four fungi except C. albicans. The components are considered to induce apoptosis-like programmed cell death via hyperproduction of reactive oxygen species. This study enhances our understanding of the antifungal activity of Kampo medicine, and may contribute to the development of new and safe antifungal therapeutics. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db137b7b5c786558bd119099f95c9779 http://link.springer.com/article/10.1038/s41598-019-38916-w |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....db137b7b5c786558bd119099f95c9779 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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