Citrus Tristeza Virus Isolates of the Same Genotype Differ in Stem Pitting Severity in Grapefruit
| العنوان: | Citrus Tristeza Virus Isolates of the Same Genotype Differ in Stem Pitting Severity in Grapefruit |
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| المؤلفون: | Coetzee B, Rachelle Bester, Glynnis Cook, Johan T. Burger, Chanel Steyn, Hans J. Maree, Rochelle de Bruyn, Johannes H. J. Breytenbach |
| المصدر: | Plant Disease. 104:2362-2368 |
| بيانات النشر: | Scientific Societies, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Citrus, Aphid, Closterovirus, Genotype, biology, Citrus tristeza virus, Plant Science, biology.organism_classification, 01 natural sciences, Virology, law.invention, 03 medical and health sciences, 030104 developmental biology, Transmission (mechanics), law, Animals, Agronomy and Crop Science, Phylogeny, Citrus paradisi, Plant Diseases, 010606 plant biology & botany |
| الوصف: | Two isolates of the T68 genotype of citrus tristeza virus (CTV) were derived from a common source, GFMS12, by single aphid transmission. These isolates, named GFMS12-8 and GFMS12-1.3, induced stem pitting with differing severity in ‘Duncan’ grapefruit (Citrus × paradisi [Macfad.]). Full-genome sequencing of these isolates showed only minor nucleotide sequence differences totaling 45 polymorphisms. Numerous nucleotide changes, in relatively close proximity, were detected in the p33 open reading frame (ORF) and the leader protease domains of ORF1a. This is the first report of full-genome characterization of CTV isolates of a single genotype, derived from the same source, but showing differences in pathogenicity. The results demonstrate the development of intragenotype heterogeneity known to occur with single-stranded RNA viruses. Identification of genetic variability between isolates showing different pathogenicity will enable interrogation of specific genome regions for potential stem pitting determinants. |
| تدمد: | 1943-7692 0191-2917 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db1c35d80ff0b2d597dcbc21ad44be3d https://doi.org/10.1094/pdis-12-19-2586-re |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....db1c35d80ff0b2d597dcbc21ad44be3d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19437692 01912917 |
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