Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK Pathway
| العنوان: | Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK Pathway |
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| المؤلفون: | Wei-Chung Chou, You-Ming Ding, Ya-Chieh Wu, Ying Chen |
| المصدر: | Cellular Physiology and Biochemistry, Vol 33, Iss 6, Pp 1888-1898 (2014) |
| بيانات النشر: | Cell Physiol Biochem Press GmbH & Co KG, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Myosin Light Chains, Time Factors, Myosin light-chain kinase, Cell Survival, Pyridines, Physiology, Blotting, Western, Biology, lcsh:Physiology, Focal adhesion, lcsh:Biochemistry, Cell Movement, Cell Line, Tumor, Glioma, Caffeine, Cell Adhesion, medicine, Animals, Humans, lcsh:QD415-436, Enzyme Inhibitors, Phosphorylation, Protein kinase A, Actin, rho-Associated Kinases, Dose-Response Relationship, Drug, lcsh:QP1-981, Focal adhesion kinase, Cell migration, Rho associated kinase, medicine.disease, Amides, Rats, Cell biology, Microscopy, Fluorescence, Cell culture, Focal Adhesion Protein-Tyrosine Kinases, Cancer research, Central Nervous System Stimulants, Paxillin, Immunostaining, Signal Transduction |
| الوصف: | Aims: Glioma is the most malignant brain tumor that has the ability to migrate and invade the CNS. In this study, we investigated the signaling mechanism of caffeine on the migration of glioma cells. Methods: The effect of caffeine on cell migration was evaluated using Transwell and wound healing assays. The expression of the focal adhesion complex as it related to cell migration was assayed using Western blotting and immunostaining. Results: Caffeine decreased the migration of rat C6 and human U87MG glioma cells and down-regulated the expression of phosphorylated focal adhesion kinase (p-FAK) and p-paxillin. Caffeine also decreased p-FAK staining at the edge of glioma cells and disassembled actin stress fibers. Additionally, caffeine elevated expression of phosphorylated myosin light chain (p-MLC), an effect that could be blocked by Y27632, a rho-associated protein kinase (ROCK) inhibitor, but not myosin light chain kinase inhibitor, ML-7. Y27632 also inhibited the caffeine-reduced expression of p-FAK and p-paxillin as well as cell migration. Conclusion: Caffeine decreased the migration of glioma cell through the ROCK-focal adhesion complex pathway; this mechanism may be useful as part of clinical therapy in the future. |
| اللغة: | English |
| تدمد: | 1421-9778 1015-8987 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db8258f2d62c024ef689fbc24e4b11b9 http://www.karger.com/Article/FullText/362966 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....db8258f2d62c024ef689fbc24e4b11b9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219778 10158987 |
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