Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models
| العنوان: | Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models |
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| المؤلفون: | Daniela V. Giacone, Luciana B. Lopes, Julia Sapienza Passos, Daniel A.G. Miranda, Erica Aparecida de Oliveira, Vanessa Franco Carvalho Dartora, Letícia V. Costa-Lotufo, Jenyffer Kelly Rocha De Matos, Silvya Stuchi Maria-Engler, Edilberto R. Silveira |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Elsevier BV, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Drug, Skin Neoplasms, Alginates, media_common.quotation_subject, 02 engineering and technology, Pharmacology, FARMACOLOGIA, Biochemistry, Chitosan, 03 medical and health sciences, chemistry.chemical_compound, Structural Biology, medicine, Humans, Cytotoxicity, Melanoma, Molecular Biology, Piperidones, Piperlongumine, Cell Proliferation, 030304 developmental biology, media_common, 0303 health sciences, Cytotoxins, General Medicine, 021001 nanoscience & nanotechnology, medicine.disease, Oleic acid, chemistry, Melanocytes, Nanoparticles, Emulsions, Nanocarriers, Skin cancer, 0210 nano-technology |
| الوصف: | Due to the limited options for topical management of skin cancer, this study aimed at developing and evaluating nanoemulsions (NE) for topical delivery of the cytotoxic agent piplartine (piperlongumine). NEs were modified with chitosan or sodium alginate, and the effects on the physicochemical properties, piplartine delivery and formulation efficacy were evaluated. The nanoemulsion droplets displayed similar size (96–112 nm), but opposite charge; the polysaccharides improved piplartine penetration into and across the skin (1.3–1.9-fold) in a similar manner, increasing the ratio “drug in the skin/receptor phase” by 1.4–1.5-fold compared to the plain NE and highlighting their relevance for cutaneous localization. Oleic acid addition to the chitosan-containing NE further increased drug penetration (~1.9–2.0-fold), as did increases in drug content from 0.5 to 1%. The cytotoxicity of piplartine was ~2.8-fold higher when the drug was incorporated in the chitosan-containing NE compared to its solution (IC50 = 14.6 μM) against melanoma cells. The effects of this nanocarrier on 3D melanoma tissues were concentration-related; at 1%, piplartine elicited marked epidermis destruction. These results support the potential applicability of the chitosan-modified nanoemulsion containing piplartine as a new strategy for local management of skin cancer. |
| تدمد: | 0141-8130 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db978ee3adbf03edbb4928c42fa1f8ca https://doi.org/10.1016/j.ijbiomac.2020.09.167 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....db978ee3adbf03edbb4928c42fa1f8ca |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01418130 |
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