Effect of Human Mesenchymal Stem Cells on the Growth of HepG2 and Hela Cells
| العنوان: | Effect of Human Mesenchymal Stem Cells on the Growth of HepG2 and Hela Cells |
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| المؤلفون: | Pengyuan Yang, Xiaohui Long, Toshimasa Uemura, Rena Matsumoto |
| المصدر: | Cell Structure and Function. 38:109-121 |
| بيانات النشر: | Japan Society for Cell Biology, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | MAPK/ERK pathway, endocrine system, Physiology, Cell, Population, Uterine Cervical Neoplasms, Cell Communication, HeLa, Phosphatidylinositol 3-Kinases, medicine, Humans, education, Molecular Biology, PI3K/AKT/mTOR pathway, Cell Proliferation, Mitogen-Activated Protein Kinase Kinases, education.field_of_study, biology, Cell growth, Liver Neoplasms, Mesenchymal stem cell, Mesenchymal Stem Cells, Hep G2 Cells, Cell Biology, General Medicine, equipment and supplies, biology.organism_classification, Coculture Techniques, Cell biology, medicine.anatomical_structure, Culture Media, Conditioned, Cancer cell, Female, HeLa Cells, Signal Transduction |
| الوصف: | Human mesenchymal stem cells (hMSCs) accumulate at carcinomas and have a great impact on cancer cell's behavior. Here we demonstrated that hMSCs could display both the promotional and inhibitive effects on growth of HepG2 and Hela cells by using the conditioned media, indirect co-culture, and cell-to-cell co-culture. Cell growth was increased following the addition of lower proportion of hMSCs while decreased by treatment of higher proportion of hMSCs. We also established a novel noninvasive label way by using internalizing quantum dots (i-QDs) for study of cell-cell contact in the co-culture, which was effective and sensitive for both tracking and distinguishing different cells population without the disturbance of cells. Furthermore, we investigated the role of hMSCs in regulation of cell growth and showed that mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) signaling pathways were involved in hMSC-mediated cell inhibition and proliferation. Our findings suggested that hMSCs regulated cancer cell function by providing a suitable environment, and the discovery from the study would provide some clues for development of effective strategy for hMSC-based cancer therapies. |
| تدمد: | 1347-3700 0386-7196 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbb9541c1dce8856110248d549271c4e https://doi.org/10.1247/csf.12029 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....dbb9541c1dce8856110248d549271c4e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13473700 03867196 |
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