Additional file 1: Figure S1. CD147 expression and stable knock-down in liver cancer cells. Figure S2. Flow cytometry analysis of CD147 level on liver cancer cell surface. Figure S3. Arf6-mediated CD147 recycling promotes Huh7 and HepG2 cell adhesion to ECM. Figure S4. Arf6-KD impaired the cell-cell aggregation of Huh7 and HepG2 cells. Arf6-perturbed cells were reseeded on agar for static culture, and cell aggregation clusters were evaluated. Figure S5. Morphometric analyses of Arf6-perturbed liver cancer cells. Figure S6. ARF6-specific GEFs and GAPs expressed in liver cancer patients. Box plots depict the expression level difference between liver cancer (T) and normal tissues (N). Figure S7. Co-expression network analysis of the Arf6-CD147 gene pair. Figure S8. Pair-wise correlation analysis for the expression (IHC staining) levels of CD147, Arf6, Rac1 and ARNO in primary HCC tissues. Table S1. Clinicopathological features of HCC patients and association with co-expression of CD147, Arf6, ARNO, and Rac1.
