Granulocyte-macrophage colony-stimulating factor protects mice against hepatocellular carcinoma by ameliorating intestinal dysbiosis and attenuating inflammation
| العنوان: | Granulocyte-macrophage colony-stimulating factor protects mice against hepatocellular carcinoma by ameliorating intestinal dysbiosis and attenuating inflammation |
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| المؤلفون: | Tuo Chen, Lei Zhang, Guangxiu Liu, Li-Hong He, Xun Li, Yong-Na Wu |
| المصدر: | World Journal of Gastroenterology |
| بيانات النشر: | Baishideng Publishing Group Inc, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Carcinoma, Hepatocellular, Hepatocellular carcinoma, Mice, Nude, Inflammation, Intestinal dysbiosis, 03 medical and health sciences, Mice, 0302 clinical medicine, RNA, Ribosomal, 16S, medicine, Animals, Microbiome, Granulocyte-macrophage colony-stimulating factor, Mice, Inbred BALB C, business.industry, Liver Neoplasms, Gastroenterology, General Medicine, Basic Study, medicine.disease, digestive system diseases, Granulocyte macrophage colony-stimulating factor, 030220 oncology & carcinogenesis, Cancer research, Dysbiosis, 030211 gastroenterology & hepatology, medicine.symptom, business, medicine.drug |
| الوصف: | BACKGROUND Hepatocellular carcinoma (HCC) is the third leading cause of cancer mortality worldwide. The gut microbiota can help maintain healthy metabolism and immunity. Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a critical factor in promoting health and homeostasis; it promotes intestinal immunity, stimulates bone marrow precursors to generate macrophage colonies, and enhances the antibacterial and antitumor activity of circulating monocytes. As such, GM-CSF may protect against HCC development by regulating immunity as well as intestinal microecology. AIM To investigate the impact of GM-CSF on the gut microbiome and metabolic characteristics of HCC. METHODS Thirty-six male BALB/c nude mice were divided into three groups: Control (n = 10), HCC (n = 13), and HCC + GM-CSF (GM-CSF overexpression, n = 13). We utilized HCC cells to establish orthotopic transplantation tumor models of HCC with normal and over-expressing GM-CSF. Liver injury, immune inflammatory function and intestinal barrier function were evaluated. The fecal microbiome and metabolome were studied using 16S rRNA absolute quantification sequencing and gas chromatography-mass spectrometry. RESULTS GM-CSF overexpression significantly affected the gut microbiome of mice with HCC and resulted in a high abundance of organisms of the genera Roseburia, Blautia and Butyricimonass, along with a significant reduction in Prevotella, Parabacteroides, Anaerotruncus, Streptococcus, Clostridium, and Mucispirillum. Likewise, GM-CSF overexpression resulted in a substantial increase in fecal biotin and oleic acid levels, along with a prominent decrease in the fecal succinic acid, adenosine, fumaric acid, lipoic acid, and maleic acid levels. Correlation analysis revealed that the intestinal microbiota and fecal metabolites induced by GM-CSF were primarily involved in pathways related to reducing the inflammatory response, biotin metabolism, and intestinal barrier dysfunction. CONCLUSION GM-CSF can protect against HCC development by regulating immunity and modulating the abundance of specific intestinal microorganisms and their metabolites. This study provides new insights into the therapeutic approaches for HCC. |
| اللغة: | English |
| تدمد: | 2219-2840 1007-9327 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc2ea6a994108bc2020b2dd315beea67 http://europepmc.org/articles/PMC7520605 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....dc2ea6a994108bc2020b2dd315beea67 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22192840 10079327 |
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