التفاصيل البيبلوغرافية
| العنوان: |
SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay |
| المؤلفون: |
Jerome Nicod, James I. MacRae, Amin Oomatia, Laura Cubitt, Iana Martini, Simon Caidan, Kiran Gulati, Sonia Gandhi, Luke Nightingale, Michael D. Buck, Enzo Z. Poirier, Wei-Ting Lu, Margaret Crawford, Nicola O’Reilly, Amy Strange, Michael Howell, Steve Hindmarsh, Charles Swanton, Jessica Olsen, Samra Turajlic, George Kassiotis, Paul Grant, Rupert Beale, Caetano Reis e Sousa, Mary Wu, Ming Jiang, Robert L. Goldstone, Sam Barrell, Eleni Nastouli, Rachael Instrell, Bruno Frederico, Davin Miller, Anett Rideg, Ana Cardoso, Ok-Ryul Song, Johnathan Canton, Mike Hubank, David Moore, Richard D. Byrne |
| المصدر: |
Wellcome Open Research |
| سنة النشر: |
2021 |
| مصطلحات موضوعية: |
0301 basic medicine, 2019-20 coronavirus outbreak, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), 030106 microbiology, Medicine (miscellaneous), General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Pandemic, Medicine, Medical physics, Viral rna, Diagnostic laboratory, Intensive care medicine, License, RT-LAMP, business.industry, SARS-CoV-2, Diagnostic test, Creative commons, Articles, Method Article, 3. Good health, 030104 developmental biology, business, clinical diagnostic |
| الوصف: |
The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARSCoV-2 detection by RT-LAMP in 25 minutes that is robust, reliable, repeatable, sensitive, specific, and inexpensive © 2021. Buck MD et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
| تدمد: |
2398-502X |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc322887fda23a44d7dc5efaf641021c
https://pubmed.ncbi.nlm.nih.gov/34095506 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.doi.dedup.....dc322887fda23a44d7dc5efaf641021c |
| قاعدة البيانات: |
OpenAIRE |
