Elimination of lipaemic interference by high-speed centrifugation
| العنوان: | Elimination of lipaemic interference by high-speed centrifugation |
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| المؤلفون: | Solé-Enrech, Gemma, Cano-Corres, Ruth, Aparicio-Calvente, Maria Isabel, Spataro, Nino |
| المصدر: | Biochemia Medica Volume 33 Issue 1 |
| بيانات النشر: | Croatian Society for Medical Biochemistry and Laboratory Medicine, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Biochemistry (medical), Clinical Biochemistry, lipaemia, high-speed centrifugation, lipaemic index |
| الوصف: | IntroductionIn order to deliver high quality results, detection and elimination of possible analytical interferences, such as lipaemia, is crucial. The aim of this study is to evaluate the efficacy of high-speed centrifugation in eliminating lipaemic interference and to define own lipaemic index (LI) for the studied biochemical analytes. Materials and methodsEvaluated analytes were: albumin, alkaline phosphatase, alanine-aminotransferase (ALT), aspartate-aminotransferase (AST), calcium, creatinine, gamma-glutamyltransferase (GGT), glucose, phosphates, total proteins, urea and total bilirubin. Those analytes and LIs have been analysed in duplicate in the Roche Diagnostics-c8000 analyser in samples centrifuged at 3000 rpm/10 minutes in the SL16 (Thermo Scientific, Waltham, USA) centrifuge and according to an own high-speed centrifugation protocol (12,900 rpm/15 minutes) in the MicroCL17R (Thermo Scientific, Waltham, USA) centrifuge. Lipaemia has been measured in each sample. The efficiency of high-speed centrifugation is verified by the Wilcoxon test (P < 0.05). In cases where significant differences are observed, our own LI is calculated. For ALT and AST, it is verified by McNemar test (P < 0.05). For creatinine, both Wilcoxon and McNemar test were applied. ResultsThere were statistically significant differences in analyte concentration before and after high-speed centrifugation for: albumin, creatinine, GGT, glucose, phosphates, urea and total bilirrubin. Own LI is calculated. McNemar test shows statistically significant diferences in the proportion of delivered results before and after high-speed centrifugation in ALT, AST and creatinine. ConclusionsThis study confirms the efficacy of high-speed centrifugation protocol for all the considered analytes, excepting calcium, alkaline phosphatase and total proteins. |
| وصف الملف: | application/pdf |
| تدمد: | 1846-7482 1330-0962 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc76d655b827a5bfee0e08291ce6d411 https://doi.org/10.11613/bm.2023.010703 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....dc76d655b827a5bfee0e08291ce6d411 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18467482 13300962 |
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