5-Hydroxytryptophan Acts on the Mitogen-Activated Protein Kinase Extracellular-Signal Regulated Protein Kinase Pathway to Modulate Cyclooxygenase-2 and Inducible Nitric Oxide Synthase Expression in RAW 264.7 Cells
| العنوان: | 5-Hydroxytryptophan Acts on the Mitogen-Activated Protein Kinase Extracellular-Signal Regulated Protein Kinase Pathway to Modulate Cyclooxygenase-2 and Inducible Nitric Oxide Synthase Expression in RAW 264.7 Cells |
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| المؤلفون: | Dong Hwan Sohn, You-Chang Oh, Hye-Jin Jang, Jang-Gi Choi, Kyu Yong Jung, Hee-Sung Chae, Dong-Yeul Kwon, Ok-Hwa Kang, Jong-Hak Kim, Young-Seob Lee, Hyun Park |
| المصدر: | Biological and Pharmaceutical Bulletin. 32:553-557 |
| بيانات النشر: | Pharmaceutical Society of Japan, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, MAPK/ERK pathway, Lipopolysaccharide, Blotting, Western, Nitric Oxide Synthase Type II, Tetrazolium Salts, Pharmaceutical Science, Enzyme-Linked Immunosorbent Assay, Nitric Oxide, Nitric oxide, 5-Hydroxytryptophan, Mice, chemistry.chemical_compound, Animals, Phosphorylation, Protein kinase A, Pain Measurement, Pharmacology, Mice, Inbred ICR, biology, Interleukin-6, Reverse Transcriptase Polymerase Chain Reaction, Akt/PKB signaling pathway, Macrophages, General Medicine, Analgesics, Non-Narcotic, Molecular biology, Nitric oxide synthase, Thiazoles, chemistry, Cyclooxygenase 2, Mitogen-activated protein kinase, biology.protein, Cyclooxygenase, Mitogen-Activated Protein Kinases, Signal Transduction |
| الوصف: | In this study, we evaluated the effect of 5-hydroxytryptophan on anti-inflammatory and analgesic activity in RAW 264.7 cells. Cells were treated with different concentrations of 5-hydroxytryptophan for either 1 h or for 24 h. The anti-inflammatory effect was then analyzed by enzyme-linked immunosorbent assay (ELISA), Western blotting and reverse transcription polymerase chain reaction (RT-PCR). In addition, the analgesic activity was evaluated by measuring the acetic acid-induced writhing response. We found that 5-hydroxytryptophan significantly reduced the acetic acid-induced writhing response. Moreover we evaluated the effects of 5-hydroxytryptophan on the release of several inflammatory mediators including nitric oxide (NO) and interleukin-6 (IL-6). Our results demonstrated that 5-hydroxytryptophan inhibited the lipopolysaccharide (LPS)-induced expression of NO and IL-6. Furthermore, we found that 5-hydroxytryptophan played a role in LPS induced inducible nitric oxide synthase (iNOS), cyclo oxygenase-2 (COX-2) and extracellular-signal regulated protein kinase (ERK) activation. Taken together, these results indicate that 5-hydroxytryptophan has the potential for use in the treatment of inflammatory disease and as an analgesic. |
| تدمد: | 1347-5215 0918-6158 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dca400b1731d2707d004bada53dbbd69 https://doi.org/10.1248/bpb.32.553 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....dca400b1731d2707d004bada53dbbd69 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13475215 09186158 |
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