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// Hong Zhou 1, 2, 5, * , Jiawei Zhang 2, 3, * , Ying Gu 1, 2, 3, * , Xiaoxian Gan 3, 4 , Yichao Gan 1, 2 , Weiwei Zheng 1, 2 , Byung-Wook Kim 3 , Xiaohua Xu 1 , Xiaoya Lu 1, 2 , Qi Dong 2 , Shu Zheng 2 , Wendong Huang 3 , Rongzhen Xu 1, 2 1 Department of Hematology, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China 2 Cancer Institute of Zhejiang University, Hangzhou 310009, China 3 Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA 91010, USA 4 Zhejiang Academy of Medical Sciences, Hangzhou 310012, China 5 Hangzhou First People’s Hospital, Hangzhou 310006, China * These authors contributed equally to this work. Correspondence to: Rongzhen Xu, e-mail: zrxyk10@zju.edu.cn Wendong Huang, e-mail: whuang@coh.org Keywords: cancer cell, RNA giant nuclear body, nuclear RNA trafficking Received: August 25, 2015 Accepted: November 25, 2015 Published: December 15, 2015 ABSTRACT Constitutive synthesis of oncogenic mRNAs is essential for maintaining the uncontrolled growth of cancer cells. However, little is known about how these mRNAs are exported from the nucleus to the cytoplasm. Here, we report the identification of a RNA giant nuclear body (RNA-GNB) that is abundant in cancer cells but rare in normal cells. The RNA-GNB contains a RNA core surrounded by a protein shell. We identify 782 proteins from cancer-associated RNA-GNBs, 40% of which are involved in the nuclear mRNA trafficking. RNA-GNB is required for cell proliferation, and its abundance is positively associated with tumor burden and outcome of therapies. Our findings suggest that the RNA-GNB is a novel nuclear RNA trafficking organelle that may contribute to the nuclear mRNA exporting and proliferation of cancer cells. |
