Interaction of human erythrocyte catalase with air–water interface in cryoEM
العنوان: | Interaction of human erythrocyte catalase with air–water interface in cryoEM |
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المؤلفون: | Shaoxia Chen, Jade Li, Kutti R Vinothkumar, Richard Henderson |
المصدر: | Microscopy. 71:i51-i59 |
بيانات النشر: | Oxford University Press (OUP), 2022. |
سنة النشر: | 2022 |
مصطلحات موضوعية: | Erythrocytes, Structural Biology, Cryoelectron Microscopy, Humans, Proteins, Water, Radiology, Nuclear Medicine and imaging, Catalase, Instrumentation |
الوصف: | One of the key goals in single-particle cryo-microscopy is to obtain a uniform distribution of particle orientations, so that the three-dimensional structure has isotropic resolution in Fourier space. A common problem arises from the interaction of protein molecules with the air–water interface that exists on both surfaces of the thin film of liquid that is formed prior to plunge-freezing into liquid ethane. Some proteins and other macromolecular complexes are disrupted by interaction with the air–water interface. Other proteins or macromolecules either become concentrated through their interaction with the interface or are excluded because they bind strongly to some other part of the grid or the filter paper used in blotting. In this paper, the interaction of human erythrocyte catalase with the air–water interface is investigated and minimized by the addition of certain detergents. Detergents can form an amphipathic monolayer at the air–water interface that creates a barrier and leaves the molecules free to adopt a variety of orientations, thus facilitating the 3D structure determination. These results suggest that further characterization and development of detergents for cryo-microscopy plunge-freezing would be useful. |
تدمد: | 2050-5701 2050-5698 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcfc3dba0defd9ebcd7eede2fa84b0c2 https://doi.org/10.1093/jmicro/dfab037 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....dcfc3dba0defd9ebcd7eede2fa84b0c2 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 20505701 20505698 |
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