Steroid-Mediated Decrease in Blood Mesenchymal Stem Cells in Liver Transplant could Impact Long-Term Recovery
| العنوان: | Steroid-Mediated Decrease in Blood Mesenchymal Stem Cells in Liver Transplant could Impact Long-Term Recovery |
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| المؤلفون: | Baburao Koneru, Dorian Wilson, Yasmine Sh Mourad, Faraz Chaudhry, Yuriy Gubenko, Lloyd Brown, Pranela Rameshwar, Jean Daniel Eloy, Steven J. Greco, Nikolaos Pyrsopoulos, Nykia D. Walker, Catherine Schoenberg, Katherine Liu, Michael Buxhoeveden, Andrei Botea, Nicholas M. Ponzio |
| المصدر: | Stem Cell Reviews and Reports. 13:644-658 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Graft Rejection, 0301 basic medicine, Receptors, CXCR4, Cancer Research, Stromal cell, Primary Cell Culture, Cell Count, Inflammation, Biology, Mesenchymal Stem Cell Transplantation, Methylprednisolone, CXCR4, Proinflammatory cytokine, End Stage Liver Disease, Andrology, Cell therapy, 03 medical and health sciences, Immune system, Cell Movement, medicine, Humans, Mesenchymal stem cell, Mesenchymal Stem Cells, Recovery of Function, Cell Biology, Chemokine CXCL12, Liver Transplantation, surgical procedures, operative, 030104 developmental biology, Gene Expression Regulation, Liver, Case-Control Studies, Immunology, medicine.symptom, Stem cell, Immunosuppressive Agents, Signal Transduction |
| الوصف: | Orthotopic liver transplant (OLT) remains the standard of care for end stage liver disease. To circumvent allo-rejection, OLT subjects receive gluococorticoids (GC). We investigated the effects of GC on endogenous mesenchymal stem (stromal) cells (MSCs) in OLT. This question is relevant because MSCs have regenerative potential and immune suppressor function. Phenotypic analyses of blood samples from 12 OLT recipients, at pre-anhepatic, anhepatic and post-transplant (2 h, Days 1 and 5) indicated a significant decrease in MSCs after GC injection. The MSCs showed better recovery in the blood from subjects who started with relatively low MSCs as compared to those with high levels at the prehepatic phase. This drop in MSCs appeared to be linked to GC since similar change was not observed in liver resection subjects. In order to understand the effects of GC on decrease MSC migration, in vitro studies were performed in transwell cultures. Untreated MSCs could not migrate towards the GC-exposed liver tissue, despite CXCR4 expression and the production of inflammatory cytokines from the liver cells. GC-treated MSCs were inefficient with respect to migration towards CXCL12, and this correlated with retracted cytoskeleton and motility. These dysfunctions were partly explained by decreases in the CXCL12/receptor axis. GC-associated decrease in MSCs in OLT recipients recovered post-transplant, despite poor migratory ability towards GC-exposed liver. In total, the study indicated that GC usage in transplant needs to be examined to determine if this could be reduced or avoided with adjuvant cell therapy. |
| تدمد: | 1558-6804 1550-8943 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd4d27bf33ea60cc58ed039f01726622 https://doi.org/10.1007/s12015-017-9751-3 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....dd4d27bf33ea60cc58ed039f01726622 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15586804 15508943 |
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