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Bacterial Adaptation in Structured Environments: Lessons from Darwin’s Finches

التفاصيل البيبلوغرافية
العنوان: Bacterial Adaptation in Structured Environments: Lessons from Darwin’s Finches
المؤلفون: Joe J. Harrison, Boo Shan Tseng, Matthew R. Parsek
المصدر: Proceedings of the National Academy of Sciences of the United States of America
Trends Microbiol
بيانات النشر: Elsevier BV, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Microbiology (medical), diversification, Burkholderia cenocepacia, Burkholderia, Gene regulatory network, Gene mutation, Microbiology, Article, biofilm, 03 medical and health sciences, Bacterial Proteins, Virology, biology.animal, Animals, experimental evolution, Cyclic GMP, Gene, Finch, 030304 developmental biology, 0303 health sciences, Virulence, Bacteria, cyclic-di-GMP, biology, 030306 microbiology, fungi, Biofilm, Quorum Sensing, Gene Expression Regulation, Bacterial, Darwin's finches, Biological Sciences, biology.organism_classification, Adaptation, Physiological, Phenotype, Infectious Diseases, Evolutionary biology, Biofilms, Mutation, Finches, Directed Molecular Evolution, Adaptation, Signal Transduction
الوصف: Significance Many organisms, including bacteria, live in fluctuating environments that require attachment and dispersal. These lifestyle decisions require processing of multiple external signals by several genetic pathways, but how they are integrated is largely unknown. We conducted multiple evolution experiments totaling >20,000 generations with Burkholderia cenocepacia populations grown in a model of the biofilm life cycle and identified parallel mutations in one gene, rpfR, that is a conserved central regulator. Because RpfR has multiple sensor and catalytic domains, different mutations can produce different ecological strategies that can coexist and even increase net growth. This study demonstrates that a single gene may coordinate complex life histories in biofilm-dwelling bacteria and that selection in defined environments can reshape niche breadth by single mutations.
Many bacteria cycle between sessile and motile forms in which they must sense and respond to internal and external signals to coordinate appropriate physiology. Maintaining fitness requires genetic networks that have been honed in variable environments to integrate these signals. The identity of the major regulators and how their control mechanisms evolved remain largely unknown in most organisms. During four different evolution experiments with the opportunist betaproteobacterium Burkholderia cenocepacia in a biofilm model, mutations were most frequently selected in the conserved gene rpfR. RpfR uniquely integrates two major signaling systems—quorum sensing and the motile–sessile switch mediated by cyclic-di-GMP—by two domains that sense, respond to, and control the synthesis of the autoinducer cis-2-dodecenoic acid (BDSF). The BDSF response in turn regulates the activity of diguanylate cyclase and phosphodiesterase domains acting on cyclic-di-GMP. Parallel adaptive substitutions evolved in each of these domains to produce unique life history strategies by regulating cyclic-di-GMP levels, global transcriptional responses, biofilm production, and polysaccharide composition. These phenotypes translated into distinct ecology and biofilm structures that enabled mutants to coexist and produce more biomass than expected from their constituents grown alone. This study shows that when bacterial populations are selected in environments challenging the limits of their plasticity, the evolved mutations not only alter genes at the nexus of signaling networks but also reveal the scope of their regulatory functions.
تدمد: 0966-842X
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de0ad4d4452a5f81cdc9306a5977421c
https://doi.org/10.1016/j.tim.2020.10.010
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....de0ad4d4452a5f81cdc9306a5977421c
قاعدة البيانات: OpenAIRE
الوصف
تدمد:0966842X