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Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approaches

التفاصيل البيبلوغرافية
العنوان: Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approaches
المؤلفون: Yanyan Chang, Junjun Shao, Guanglei Zhang, Huiyun Chang, Lei Xiao, Sudan Ge, Zhan Gao
المصدر: Virology Journal
Virology Journal, Vol 18, Iss 1, Pp 1-10 (2021)
بيانات النشر: Springer Science and Business Media LLC, 2021.
سنة النشر: 2021
مصطلحات موضوعية: 0301 basic medicine, Recombinant protein, Multi-epitope, Swine, 030106 microbiology, Enzyme-Linked Immunosorbent Assay, Infectious and parasitic diseases, RC109-216, Antibodies, Viral, Bioinformatics, African swine fever virus, Epitope, law.invention, Epitopes, 03 medical and health sciences, Antigen, law, Indirect enzyme-linked immunosorbent assay, Virology, Animals, African Swine Fever, Gene, biology, Methodology, Computational Biology, Reproducibility of Results, biology.organism_classification, African Swine Fever Virus, Fusion protein, Recombinant Proteins, 030104 developmental biology, Infectious Diseases, Infectious disease (medical specialty), biology.protein, Recombinant DNA, Antibody
الوصف: Background African swine fever (ASF), characterized by acute, severe, and fast-spreading, is a highly lethal swine infectious disease caused by the African swine fever virus (ASFV), which has caused substantial economic losses to the pig industry worldwide in the past 100 years. Methods This study started with bioinformatics methods and verified the epitope fusion protein method's reliability that does not rely on traditional epitope identification. Meanwhile, it will also express and purify the constructed genes through prokaryotic expression and establish antibody detection methods. Results The results indicated that the protein had good reactivity and did not cross-react with other swine diseases. The receiver-operating characteristic analysis was performed to verify the determination. The area under the receiver-operating characteristic curve was 0.9991 (95% confidence interval 0.9973 to 1.001). Conclusions It was proved that the recombinant protein is feasible as a diagnostic antigen to distinguish ASFV and provides a new idea for ASFV antibody detection.
تدمد: 1743-422X
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df4759fd8d256ff67eb6d8f022193f1f
https://doi.org/10.1186/s12985-021-01568-2
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....df4759fd8d256ff67eb6d8f022193f1f
قاعدة البيانات: OpenAIRE
الوصف
تدمد:1743422X