Molecular Cloning of ATR5Emoy2 from Hyaloperonospora arabidopsidis, an Avirulence Determinant That Triggers RPP5-Mediated Defense in Arabidopsis
| العنوان: | Molecular Cloning of ATR5Emoy2 from Hyaloperonospora arabidopsidis, an Avirulence Determinant That Triggers RPP5-Mediated Defense in Arabidopsis |
|---|---|
| المؤلفون: | Nicholas Holton, Alison Woods-Tör, Jim Beynon, Kate Bailey, Jonathan D. G. Jones, Laura Baxter, Linda Karen Hughes, Jane Byrne-Richardson, Eric B. Holub, Mahmut Tör, Kee Hoon Sohn, Volkan Cevik, Mary Coates, H Murat Aksoy |
| المساهمون: | OMÜ |
| المصدر: | Molecular Plant-Microbe Interactions®. 24:827-838 |
| بيانات النشر: | Scientific Societies, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Signal peptide, Physiology, Sequence analysis, Molecular Sequence Data, Arabidopsis, Gene Expression Regulation, Plant, Gene Duplication, Amino Acid Sequence, Amplified Fragment Length Polymorphism Analysis, Cloning, Molecular, Gene, Disease Resistance, Plant Diseases, RGD motif, Genetics, Hyaloperonospora arabidopsidis, Base Sequence, biology, Arabidopsis Proteins, Effector, Gene Expression Profiling, Proteins, Sequence Analysis, DNA, General Medicine, biology.organism_classification, Open reading frame, Genes, Oomycetes, Multigene Family, Sequence Alignment, Agronomy and Crop Science |
| الوصف: | RPP5 is the seminal example of a cytoplasmic NB-LRR receptor-like protein that confers downy mildew resistance in Arabidopsis thaliana. In this study, we describe the cloning and molecular characterization of the gene encoding ATR5Emoy2, an avirulence protein from the downy mildew pathogen Hyaloperonospora arabidopsidis isolate Emoy2. ATR5Emoy2 triggers defense response in host lines expressing the functional RPP5 allele from Landsberg erecta (Ler-0). ATR5Emoy2 is embedded in a cluster with two additional ATR5-like (ATR5L) genes, most likely resulting from gene duplications. ATR5L proteins do not trigger RPP5-mediated resistance and the copy number of ATR5L genes varies among H. arabidopsidis isolates. ATR5Emoy2 and ATR5L proteins contain a signal peptide, canonical EER motif, and an RGD motif. However, they lack the canonical translocation motif RXLR, which characterizes most oomycete effectors identified so far. The signal peptide and the N-terminal regions including the EER motif of ATR5Emoy2 are not required to trigger an RPP5-dependent immune response. Bioinformatics screen of H. arabidopsidis Emoy2 genome revealed the presence of 173 open reading frames that potentially encode for secreted proteins similar to ATR5Emoy2, in which they share some motifs such as EER but there is no canonical RXLR motif. |
| تدمد: | 1943-7706 0894-0282 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df865f3bdebe7825c544d294e780abc3 https://doi.org/10.1094/mpmi-12-10-0278 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....df865f3bdebe7825c544d294e780abc3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19437706 08940282 |
|---|