The intestinal virome in children with cystic fibrosis differs from healthy controls
| العنوان: | The intestinal virome in children with cystic fibrosis differs from healthy controls |
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| المؤلفون: | Ivan Low, Chee Y. Ooi, Sacha Stelzer-Braid, Michael J. Coffey, William D. Rawlinson, Torsten Thomas, Adam Jaffe, Bernd Wemheuer, Millie Garg |
| المصدر: | PLoS ONE, Vol 15, Iss 5, p e0233557 (2020) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Cystic Fibrosis, Pulmonology, medicine.disease_cause, Pathology and Laboratory Medicine, Cystic fibrosis, Families, Feces, Medicine and Health Sciences, Bacteriophages, Prospective Studies, Child, Children, Immune Response, Data Management, Gastrointestinal tract, Multidisciplinary, Ecology, Viral Taxonomy, Intestines, Shannon Index, Genetic Diseases, Child, Preschool, Viruses, Medicine, Female, Anatomy, Research Article, Microbial Taxonomy, Computer and Information Sciences, Ecological Metrics, Science, 030106 microbiology, Immunology, Biology, Microbiology, 03 medical and health sciences, Signs and Symptoms, Autosomal Recessive Diseases, Diagnostic Medicine, Virology, medicine, Humans, Human virome, Gene, Taxonomy, Clinical Genetics, Inflammation, Ecology and Environmental Sciences, Organisms, Biology and Life Sciences, Species Diversity, medicine.disease, biology.organism_classification, Fibrosis, Gastrointestinal Tract, 030104 developmental biology, Age Groups, Case-Control Studies, People and Places, Enterovirus, Picornavirales, Dysbiosis, Population Groupings, Exocrine Pancreatic Insufficiency, Calprotectin, Digestive System, Developmental Biology |
| الوصف: | Intestinal bacterial dysbiosis is evident in children with cystic fibrosis (CF) and intestinal viruses may be contributory, given their influence on bacterial species diversity and biochemical cycles. We performed a prospective, case-control study on children with CF and age and gender matched healthy controls (HC), to investigate the composition and function of intestinal viral communities. Stool samples were enriched for viral DNA and RNA by viral extraction, random amplification and purification before sequencing (Illumina MiSeq). Taxonomic assignment of viruses was performed using Vipie. Functional annotation was performed using Virsorter. Inflammation was measured by calprotectin and M2-pyruvate kinase (M2-PK). Eight CF and eight HC subjects were included (50% male, mean age 6.9 ± 3.0 and 6.4 ± 5.3 years, respectively, p = 0.8). All CF subjects were pancreatic insufficient. Regarding the intestinal virome, no difference in Shannon index between CF and HC was identified. Taxonomy-based beta-diversity (presence-absence Bray-Curtis dissimilarity) was significantly different between CF and HC (R2 = 0.12, p = 0.001). Myoviridae, Faecalibacterium phage FP Taranis and unclassified Gokushovirinae were significantly decreased in CF compared with HC (q |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfc31ffc8cf9991bbf1f377d67d3ee16 https://doaj.org/article/1b4788400dab4b23be75642ad0d102a2 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....dfc31ffc8cf9991bbf1f377d67d3ee16 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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