Vascular endothelial-cadherin tyrosine phosphorylation in angiogenic and quiescent adult tissues.: VE-cadherin tyrosine phosphorylation
| العنوان: | Vascular endothelial-cadherin tyrosine phosphorylation in angiogenic and quiescent adult tissues.: VE-cadherin tyrosine phosphorylation |
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| المؤلفون: | Francine Cand, Isabelle Vilgrain, Danielle Gulino-Debrac, Yann Wallez, Nathalie Lambeng, Philippe Huber, Christine Rampon, Georges Christe |
| المساهمون: | Laboratoire de développement et vieillissement de l'endothélium, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Circulation Research Circulation Research, 2005, 96 (3), pp.384-91. ⟨10.1161/01.RES.0000156652.99586.9f⟩ Circulation Research, American Heart Association, 2005, 96 (3), pp.384-91. ⟨10.1161/01.RES.0000156652.99586.9f⟩ |
| بيانات النشر: | HAL CCSD, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Cell Extracts, Umbilical Veins, Physiology, Angiogenesis, Gonadotropins, Equine, MESH: Cadherins, chemistry.chemical_compound, Mice, angiogenesis, 0302 clinical medicine, VE-cadherin, MESH: Cell Extracts, MESH: Animals, MESH: Proteins, MESH: Endothelial Cells, Tyrosine, Phosphorylation, 0303 health sciences, tyrosine kinase, Cadherins, Cell biology, 030220 oncology & carcinogenesis, MESH: Uterus, Female, MESH: Endothelium, Vascular, Cardiology and Cardiovascular Medicine, Tyrosine kinase, MESH: Neovascularization, Physiologic, Proto-oncogene tyrosine-protein kinase Src, MESH: Phosphotyrosine, MESH: Ovary, medicine.medical_specialty, endothelium, Proto-Oncogene Proteins pp60(c-src), Neovascularization, Physiologic, MESH: Umbilical Veins, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Article, Cell Line, 03 medical and health sciences, MESH: Mice, Inbred C57BL, MESH: Gonadotropins, Equine, Internal medicine, medicine, Animals, Humans, Phosphotyrosine, MESH: Mice, 030304 developmental biology, MESH: Humans, MESH: Phosphorylation, MESH: Vascular Endothelial Growth Factor Receptor-2, Ovary, Uterus, Endothelial Cells, Proteins, Kinase insert domain receptor, Tyrosine phosphorylation, MESH: Proto-Oncogene Proteins pp60(c-src), Vascular Endothelial Growth Factor Receptor-2, MESH: Cell Line, Mice, Inbred C57BL, Endocrinology, chemistry, MESH: Vanadates, Endothelium, Vascular, Vanadates, MESH: Female |
| الوصف: | International audience; Vascular endothelial-cadherin (VE-cadherin) plays a key role in angiogenesis and in vascular permeability. The regulation of its biological activity may be a central mechanism in normal or pathological angiogenesis. VE-cadherin has been shown to be phosphorylated on tyrosine in vitro under various conditions, including stimulation by VEGF. In the present study, we addressed the question of the existence of a tyrosine phosphorylated form of VE-cadherin in vivo, in correlation with the quiescent versus angiogenic state of adult tissues. Phosphorylated VE-cadherin was detected in mouse lung, uterus, and ovary but not in other tissues unless mice were injected with peroxovanadate to block protein phosphatases. Remarkably, VE-cadherin tyrosine phosphorylation was dramatically increased in uterus and ovary, and not in other organs, during PMSG/hCG-induced angiogenesis. In parallel, we observed an increased association of VE-cadherin with Flk1 (VEGF receptor 2) during hormonal angiogenesis. Additionally, Src kinase was constitutively associated with VE-cadherin in both quiescent and angiogenic tissues and increased phosphorylation of VE-cadherin-associated Src was detected in uterus and ovary after hormonal treatment. Src-VE-cadherin association was detected in cultured endothelial cells, independent of VE-cadherin phosphorylation state and Src activation level. In this model, Src inhibition impaired VEGF-induced VE-cadherin phosphorylation, indicating that VE-cadherin phosphorylation was dependent on Src activation. We conclude that VE-cadherin is a substrate for tyrosine kinases in vivo and that its phosphorylation, together with that of associated Src, is increased by angiogenic stimulation. Physical association between Flk1, Src, and VE-cadherin may thus provide an efficient mechanism for amplification and perpetuation of VEGF-stimulated angiogenic processes. |
| اللغة: | English |
| تدمد: | 0009-7330 1524-4571 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e02ffabb1212021940bbd2dea56b8c03 https://www.hal.inserm.fr/inserm-00433466/file/Lambeng_Fig_4.pdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e02ffabb1212021940bbd2dea56b8c03 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00097330 15244571 |
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