Pentatricopeptide repeat protein MITOCHONDRIAL STABILITY FACTOR 3 ensures mitochondrial RNA stability and embryogenesis
| العنوان: | Pentatricopeptide repeat protein MITOCHONDRIAL STABILITY FACTOR 3 ensures mitochondrial RNA stability and embryogenesis |
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| المؤلفون: | Chuande Wang, Lisa Blondel, Martine Quadrado, Céline Dargel-Graffin, Hakim Mireau |
| المصدر: | Plant Physiol |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Arabidopsis Proteins, RNA, Mitochondrial, Physiology, RNA Splicing, Arabidopsis, Embryonic Development, Focus Issue on Evolution of Plant Structure and Function, Plant Science, Introns, Mitochondria, Mitochondrial Proteins, Gene Expression Regulation, Plant, Genetics, RNA, Messenger, Plant Proteins |
| الوصف: | Gene expression in plant mitochondria is predominantly governed at the post-transcriptional level and relies mostly on nuclear-encoded proteins. However, the protein factors involved and the underlying molecular mechanisms are still not well understood. Here, we report on the function of the MITOCHONDRIAL STABILITY FACTOR 3 (MTSF3) protein, previously named EMBRYO DEFECTIVE 2794 (EMB2794), and show that it is essential for accumulation of the mitochondrial NADH dehydrogenase subunit 2 (nad2) transcript in Arabidopsis (Arabidopsis thaliana) but not for splicing of nad2 intron 2 as previously proposed. The MTSF3 gene encodes a pentatricopeptide repeat protein that localizes in the mitochondrion. An MTSF3 null mutation induces embryonic lethality, but viable mtsf3 mutant plants can be generated through partial complementation with the developmentally regulated ABSCISIC ACID INSENSITIVE3 promoter. Genetic analyses revealed growth retardation in rescued mtsf3 plants owing to the specific destabilization of mature nad2 mRNA and a nad2 precursor transcript bearing exons 3 to 5. Biochemical data demonstrate that MTSF3 protein specifically binds to the 3′ terminus of nad2. Destabilization of nad2 mRNA induces a substantial decrease in complex I assembly and activity and overexpression of the alternative respiratory pathway. Our results support a role for MTSF3 protein in protecting two nad2 transcripts from degradation by mitochondrial exoribonucleases by binding to their 3′ extremities. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e077bca0db123bc610becefb686a1232 https://doi.org/10.1093/plphys/kiac309 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e077bca0db123bc610becefb686a1232 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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