Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men
| العنوان: | Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men |
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| المؤلفون: | Ljiljana Hajduković, Irena Miler, Tamara Janković, Jelena Danilović Luković, Ninoslav Mitić, Miroslava Janković |
| المصدر: | Upsala Journal of Medical Sciences Upsala Journal of Medical Sciences, Vol 126, Pp 1-10 (2021) |
| بيانات النشر: | Open Academia, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Glycan, Tetraspanins, Galectin 3, normozoospermia, Polysaccharides, Semen, detergent sensitivity, CD63, Extracellular, Medicine, Humans, Integral membrane protein, chemistry.chemical_classification, biology, business.industry, Vesicle, Vesicle coat, General Medicine, Extracellular vesicles, CD9, Spermatozoa, oligozoospermia, Biochemistry, chemistry, Concanavalin A, biology.protein, Prostasomes, Original Article, gamma-glutamyl transferase, Glycoprotein, business, molecular patterns |
| الوصف: | Background: Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes. In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease. Methods: Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that maintained complete solubilization. Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot. Results: Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat. In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3. Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins. Conclusions: Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements. |
| اللغة: | English |
| تدمد: | 2000-1967 0300-9734 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e09a107be01297bd7e9156a7c50c937b http://europepmc.org/articles/PMC8431989 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e09a107be01297bd7e9156a7c50c937b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20001967 03009734 |
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