The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II
العنوان: | The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II |
---|---|
المؤلفون: | Martijn S. Luijsterburg, Johannes C. Walter, Sheera Adar, Hadar Golan-Berman, Diana van den Heuvel, Román González-Prieto, Joachim Goedhart, Elisheva E. Heilbrun, Katja Apelt, Alfred C.O. Vertegaal, Yana van der Weegen, Tycho E. T. Mevissen |
المساهمون: | Molecular Cytology (SILS, FNWI) |
المصدر: | Nature Communications, Vol 11, Iss 1, Pp 1-16 (2020) Nature Communications, 11:2104. Nature Publishing Group Nature Communications Nature Communications, 11(1). NATURE PUBLISHING GROUP |
بيانات النشر: | Nature Portfolio, 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | 0301 basic medicine, DNA repair, DNA damage, Science, General Physics and Astronomy, RNA polymerase II, chemical and pharmacologic phenomena, Biology, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, 0302 clinical medicine, DNA repair complex, Transcription (biology), lcsh:Science, Multidisciplinary, T-cell receptor, DNA damage and repair, hemic and immune systems, General Chemistry, Cell biology, Nucleotide excision repair, 030104 developmental biology, Transcription Factor TFIIH, Transcription factor II H, biology.protein, lcsh:Q, Transcription, 030217 neurology & neurosurgery |
الوصف: | The response to DNA damage-stalled RNA polymerase II (RNAPIIo) involves the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. The function of the TCR proteins CSB, CSA and UVSSA and the manner in which the core DNA repair complex, including transcription factor IIH (TFIIH), is recruited are largely unknown. Here, we define the assembly mechanism of the TCR complex in human isogenic knockout cells. We show that TCR is initiated by RNAPIIo-bound CSB, which recruits CSA through a newly identified CSA-interaction motif (CIM). Once recruited, CSA facilitates the association of UVSSA with stalled RNAPIIo. Importantly, we find that UVSSA is the key factor that recruits the TFIIH complex in a manner that is stimulated by CSB and CSA. Together these findings identify a sequential and highly cooperative assembly mechanism of TCR proteins and reveal the mechanism for TFIIH recruitment to DNA damage-stalled RNAPIIo to initiate repair. The response to DNA damage-stalled RNA polymerase II leads to the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. Here, the authors reveal the complex assembly mechanism of the TCR complex in human cells. |
وصف الملف: | application/pdf |
اللغة: | English |
تدمد: | 2041-1723 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0b58bed0c87436f678cf7581c50542f https://doaj.org/article/0f49222ee301497fadd3b4e75734a6d9 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....e0b58bed0c87436f678cf7581c50542f |
قاعدة البيانات: | OpenAIRE |
تدمد: | 20411723 |
---|