Porous Polymeric Microspheres With Controllable Pore Diameters for Tissue Engineered Lung Tumor Model Development
| العنوان: | Porous Polymeric Microspheres With Controllable Pore Diameters for Tissue Engineered Lung Tumor Model Development |
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| المؤلفون: | Rachel Movsas, Dinesh Dhamecha, Duong Le, Jyothi U. Menon, Andrea Gonsalves |
| المصدر: | Frontiers in Bioengineering and Biotechnology Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Histology, lcsh:Biotechnology, Cell, Biomedical Engineering, Bioengineering, 02 engineering and technology, scaffold, 03 medical and health sciences, chemistry.chemical_compound, controlled pore formation, In vivo, lcsh:TP248.13-248.65, Monolayer, medicine, tissue engineered, drug screening, Porosity, Fibroblast, Original Research, chemistry.chemical_classification, microparticles, Bioengineering and Biotechnology, Polymer, 021001 nanoscience & nanotechnology, In vitro, PLGA, 030104 developmental biology, medicine.anatomical_structure, chemistry, lung cancer co-culture, 0210 nano-technology, Biotechnology, Biomedical engineering |
| الوصف: | Complex cell cultures are more representative of in vivo conditions than conventionally used monolayer cultures, and are hence being investigated for predictive screening of therapeutic agents. Poly lactide co-glycolide (PLGA) polymer is frequently used in the development of porous substrates for complex cell culture. Substrates or scaffolds with highly interconnected, micrometric pores have been shown to positively impact tissue model formation by enhancing cell attachment and infiltration. We report a novel alginate microsphere (AMS)-based controlled pore formation method for the development of porous, biodegradable PLGA microspheres (PPMS), for tissue engineered lung tumor model development. The AMS porogen, non-porous PLGA microspheres (PLGAMS) and PPMS had spherical morphology (mean diameters: 10.3 ± 4, 79 ± 21.8, and 103 ± 30 μm, respectively). The PPMS had relatively uniform pores and a porosity of 45.5%. Degradation studies show that PPMS effectively maintained their structural integrity with time whereas PLGAMS showed shrunken morphology. The optimized cell seeding density on PPMS was 25 × 103 cells/mg of particles/well. Collagen coating on PPMS significantly enhanced the attachment and proliferation of co-cultures of A549 lung adenocarcinoma and MRC-5 lung fibroblast cells. Preliminary proof-of-concept drug screening studies using mono- and combination anti-cancer therapies demonstrated that the tissue-engineered lung tumor model had a significantly higher resistance to the tested drugs than the monolayer co-cultures. These studies indicate that the PPMS with controllable pore diameters may be a suitable platform for the development of complex tumor cultures for early in vitro drug screening applications. |
| اللغة: | English |
| تدمد: | 2296-4185 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0bf5479c34016cd5817aebe7eea6281 http://europepmc.org/articles/PMC7365955 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e0bf5479c34016cd5817aebe7eea6281 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22964185 |
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