Recovery of high value‐added protein from enzyme‐assisted aqueous extraction (EAE ) of soybeans by dead‐end ultrafiltration
| العنوان: | Recovery of high value‐added protein from enzyme‐assisted aqueous extraction ( |
|---|---|
| المؤلفون: | Yang Li, Qiaozhi Zhang, Zhongjiang Wang, Sui Xiaonan, Lianzhou Jiang, Baokun Qi |
| المصدر: | Food Science & Nutrition Food Science & Nutrition, 7(2):858-868 Food Science & Nutrition, Vol 7, Iss 2, Pp 858-868 (2019) |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | protein rejection coefficient, 030309 nutrition & dietetics, Ultrafiltration, lcsh:TX341-641, Fraction (chemistry), nutrition value, law.invention, 03 medical and health sciences, 0404 agricultural biotechnology, enzyme‐assisted aqueous extraction, law, skim fraction, functional property, ultrafiltration, medicine, Filtration, Original Research, chemistry.chemical_classification, 0303 health sciences, Aqueous solution, Chromatography, Chemistry, Extraction (chemistry), 04 agricultural and veterinary sciences, Trypsin, 040401 food science, Enzyme, Membrane, lcsh:Nutrition. Foods and food supply, Food Science, medicine.drug |
| الوصف: | The skim fraction (SF) obtained from enzyme‐assisted aqueous extraction (EAE) of soybeans is a by‐product with high protein content of up to 60.67%. As such, it is of great interest to develop an efficient method to recover protein from this fraction. In this study, the potential of dead‐end ultrafiltration (UF) in recovering skim protein extracted with different proteases was evaluated. Two polyethersulfone (PES) membranes with molecular weight cutoffs (MWCO) of 3 kDa and 5 kDa were utilized. Results revealed that the membrane with the MWCO of 5 kDa exhibited better filtration efficiency, since higher permeate flux values and lower impurity rejections were observed. Compared with Flavourzyme and Protex 7L, Alcalase 2.4L and Protex 6L exhibited stronger hydrolyzing ability, resulting in higher filtration fluxes but lower protein rejection coefficients. The recovered protein showed comparable amino acid profile to SPC, while with significantly reduced levels of trypsin inhibitors and phytate (p |
| تدمد: | 2048-7177 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0c4f5594a151d698f26e64113a5a486 https://doi.org/10.1002/fsn3.936 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e0c4f5594a151d698f26e64113a5a486 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20487177 |
|---|