Cytochrome P4501A Induction by 2,3,7,8-Tetrachlorodibenzo-p-Dioxin and Two Chlorinated Dibenzofurans in Primary Hepatocyte Cultures of Three Avian Species
| العنوان: | Cytochrome P4501A Induction by 2,3,7,8-Tetrachlorodibenzo-p-Dioxin and Two Chlorinated Dibenzofurans in Primary Hepatocyte Cultures of Three Avian Species |
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| المؤلفون: | Steve Wiseman, Yi Wan, Matthew J. Zwiernik, Stephanie P. Jones, Doug Crump, John P. Giesy, Lukas J. Mundy, Steven J. Bursian, Paul D. Jones, Jessica C. Hervé, Sean W. Kennedy |
| المصدر: | Toxicological Sciences. 113:380-391 |
| بيانات النشر: | Oxford University Press (OUP), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | medicine.medical_specialty, Polychlorinated Dibenzodioxins, animal structures, Cytochrome, Coturnix, Toxicology, Pheasant, Birds, Internal medicine, biology.animal, Cytochrome P-450 CYP1A1, medicine, Animals, RNA, Messenger, Galliformes, Cells, Cultured, Benzofurans, Messenger RNA, biology, Coturnix japonica, food and beverages, biology.organism_classification, In vitro, Quail, medicine.anatomical_structure, Endocrinology, Enzyme Induction, Hepatocyte, embryonic structures, Hepatocytes, biology.protein, Aryl Hydrocarbon Hydroxylases, Chickens, Phasianus |
| الوصف: | Relative potencies of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachlorodibenzofuran (PeCDF), and 2,3,7, 8-tetrachlorodibenzofuran (TCDF) were determined in vitro in primary hepatocyte cultures of chicken (Gallus gallus), ring-necked pheasant (Phasianus colchicus), and Japanese quail (Coturnix japonica) embryos. Concentration-dependent effects on ethoxyresorufin O-deethylase (EROD) activity and expression of cytochrome P4501A4 and cytochrome P4501A5 (CYP1A4 and CYP1A5) messenger RNA (mRNA) were determined in hepatocytes exposed to serial dilutions of TCDD, PeCDF, or TCDF for 24 h. In chicken hepatocytes, the three compounds were equipotent inducers of EROD activity and CYP1A4/CYP1A5 mRNA expression. However, in ring-necked pheasant and Japanese quail hepatocytes, PeCDF was more potent than TCDD (3- to 5-fold in ring-necked pheasant and 13- to 30-fold in Japanese quail). Among species, the rank order of sensitivity (most to least) to EROD and CYP1A4/CYP1A5 mRNA induction for TCDD and TCDF was chicken > ring-necked pheasant > Japanese quail. In contrast, the three species were approximately equisensitive to EROD and CYP1A4/CYP1A5 mRNA induction by PeCDF. It has generally been assumed that TCDD is the most potent "dioxin-like compound" (DLC) and that the chicken is the most sensitive avian species to CYP1A induction by all DLCs. This study indicates that PeCDF is more potent than TCDD in ring-necked pheasant and Japanese quail hepatocytes and that ring-necked pheasant, Japanese quail, and chicken hepatocytes are equally sensitive to CYP1A induction by PeCDF. |
| تدمد: | 1096-0929 1096-6080 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0c9d8985778f2a95c68f0c0faa320b6 https://doi.org/10.1093/toxsci/kfp270 |
| رقم الأكسشن: | edsair.doi.dedup.....e0c9d8985778f2a95c68f0c0faa320b6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10960929 10966080 |
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