Metabolomic Profiling of the Synergistic Effects of Ginsenoside Rg1 in Combination with Neural Stem Cell Transplantation in Ischemic Stroke Rats
| العنوان: | Metabolomic Profiling of the Synergistic Effects of Ginsenoside Rg1 in Combination with Neural Stem Cell Transplantation in Ischemic Stroke Rats |
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| المؤلفون: | Yin-Chu Si, Caixia Jia, Jianxin Chen, Tieshan Wang, Jian Gao, Jingzhong Li, Haibin Zhao, Peng Bai, Yuanyuan Li |
| المصدر: | Journal of Proteome Research. 19:2676-2688 |
| بيانات النشر: | American Chemical Society (ACS), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, China, Ginsenosides, Traditional Chinese medicine, Pharmacology, Biochemistry, Brain Ischemia, 03 medical and health sciences, Metabolomics, Neural Stem Cells, Animals, Medicine, Stroke, Ischemic Stroke, 030102 biochemistry & molecular biology, Mechanism (biology), business.industry, General Chemistry, Metabolism, medicine.disease, Neural stem cell, Rats, Transplantation, 030104 developmental biology, Stem cell, business, Stem Cell Transplantation |
| الوصف: | As the greatest medical and socioeconomic problem in developing countries, stroke is the second or third leading cause of death in China and worldwide. In adult organisms suffering from stroke, transplanted stem cells have the ability to repair damaged tissues by regenerating autologous cells, while ginsenoside Rg1 could promote proliferation and differentiation of stem cells. Although obvious antistroke effects of ginsenoside Rg1 and transplanted stem cells have been verified in publications, the mechanism exploration remains challenging. Our study was carried out to investigate the synergistic effects of ginsenoside Rg1 and neural stem cell (NSC) transplantation on MCAO rats with a 1H NMR-based nontargeted metabolomics method to identify potential biomarkers and protein targets and discover the potential mechanism. NSCs transplantation after MCAO combined with ginsenoside Rg1 administration could significantly improve the cerebral infarct and neurological deficits. The treatment significantly intervened the levels of ten metabolites, and perturbed energy metabolism, amino acids metabolism, and lipids metabolism. And 11 enzymes were identified and verified as the targets of NSCs transplantation and ginsenoside Rg1 administration on MCAO rats. Our findings helped to improve the antistroke mechanism of NSCs transplantation and ginsenoside Rg1 and supply a theory basis for the combined research of stem cells and Chinese medicine in the future. |
| تدمد: | 1535-3907 1535-3893 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e120575f4836c8875b1a8c6a4e4408b4 https://doi.org/10.1021/acs.jproteome.9b00639 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....e120575f4836c8875b1a8c6a4e4408b4 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15353907 15353893 |
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