Knockdown of the Plasmodium falciparum SURFIN4.1 antigen leads to an increase of its cognate transcript
| العنوان: | Knockdown of the Plasmodium falciparum SURFIN4.1 antigen leads to an increase of its cognate transcript |
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| المؤلفون: | Tatiane Macedo-Silva, Wesley Luzetti Fotoran, Mauro F Azevedo, Gerhard Wunderlich, Kamila Anna Meissner, Márcia Melo Medeiros, Rosana Beatriz Duque Araujo |
| المصدر: | PLoS ONE PLoS ONE, Vol 12, Iss 8, p e0183129 (2017) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Public Library of Science, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Plasmodium, Erythrocytes, PARASITOLOGIA, Physiology, RNA Stability, lcsh:Medicine, Biochemistry, Ligases, 0302 clinical medicine, Transcription (biology), Immune Physiology, Medicine and Health Sciences, Protein Isoforms, lcsh:Science, Genetics, Regulation of gene expression, Protozoans, Feedback, Physiological, Gene knockdown, Multidisciplinary, Immune System Proteins, biology, Messenger RNA, Malarial Parasites, Antigenic Variation, Transmembrane protein, Body Fluids, Enzymes, Nucleic acids, Blood, Gene Knockdown Techniques, Anatomy, Research Article, Half-Life, Cloning, Organism, Morpholines, 030231 tropical medicine, DNA transcription, Immunology, Plasmodium falciparum, Antigens, Protozoan, Research and Analysis Methods, Transfection, 03 medical and health sciences, Parasite Groups, Gene family, Humans, RNA, Messenger, Antigens, Molecular Biology Techniques, Gene, Molecular Biology, Alleles, Life Cycle Stages, lcsh:R, Organisms, RNA, Biology and Life Sciences, Proteins, biology.organism_classification, Parasitic Protozoans, 030104 developmental biology, Gene Expression Regulation, Enzymology, lcsh:Q, Parasitology, Gene expression, Apicomplexa, Cloning |
| الوصف: | The genome of the malaria parasite Plasmodium falciparum contains the surf gene family which encodes large transmembrane proteins of unknown function. While some surf alleles appear to be expressed in sexual stages, others occur in asexual blood stage forms and may be associated to virulence-associated processes and undergo transcriptional switching. We accessed the transcription of surf genes along multiple invasions by real time PCR. Based on the observation of persistent expression of gene surf4.1, we created a parasite line which expresses a conditionally destabilized SURFIN4.1 protein. Upon destabilization of the protein, no interference of parasite growth or morphological changes were detected. However, we observed a strong increase in the transcript quantities of surf4.1 and sometimes of other surf genes in knocked-down parasites. While this effect was reversible when SURFIN4.1 was stabilized again after a few days of destabilization, longer destabilization periods resulted in a transcriptional switch away from surf4.1. When we tested if a longer transcript half-life was responsible for increased transcript detection in SURFIN4.1 knocked-down parasites, no alteration was found compared to control parasite lines. This suggests a specific feedback of the expressed SURFIN protein to its transcript pointing to a novel type of regulation, inedited in Plasmodium. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1e669c0c9b0de48ab9c7af58b6f7418 http://europepmc.org/articles/PMC5553854 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e1e669c0c9b0de48ab9c7af58b6f7418 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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