Proteome‐Wide Survey of Cysteine Oxidation by Using a Norbornene Probe
| العنوان: | Proteome‐Wide Survey of Cysteine Oxidation by Using a Norbornene Probe |
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| المؤلفون: | Marc Remke, Justin M. Chalker, Michael V. Perkins, Maike Langini, Kai Stühler, Lisa J Alcock, Gonçalo J. L. Bernardes |
| المصدر: | ChemBioChem. 21:1329-1334 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Proteome, 010402 general chemistry, Proteomics, medicine.disease_cause, 01 natural sciences, Biochemistry, Redox, Sulfenic Acids, chemistry.chemical_compound, medicine, Humans, Cysteine, Hydrogen peroxide, Molecular Biology, chemistry.chemical_classification, Reactive oxygen species, 010405 organic chemistry, Chemistry, Organic Chemistry, Norbornanes, 0104 chemical sciences, Oxidative Stress, Molecular Medicine, Sulfenic acid, Oxidation-Reduction, Oxidative stress, HeLa Cells, Signal Transduction |
| الوصف: | Rapid detection of cysteine oxidation in living cells is critical in advancing our understanding of responses to reactive oxygen species (ROS) and oxidative stress. Accordingly, there is a need to develop chemical probes that facilitate proteome-wide detection of cysteine's many oxidation states. Herein, we report the first whole-cell proteomics analysis using a norbornene probe to detect the initial product of cysteine oxidation: cysteine sulfenic acid. The oxidised proteins identified in the HeLa cell model represent the first targets of the ROS hydrogen peroxide. The panel of protein hits provides new and important information about the targets of oxidative stress, including 148 new protein members of the sulfenome. These findings provide new leads for the study and understanding of redox signalling and diseases associated with oxidative stress. |
| تدمد: | 1439-7633 1439-4227 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e208d51652f9a0f959da409f4c801b38 https://doi.org/10.1002/cbic.201900729 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e208d51652f9a0f959da409f4c801b38 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14397633 14394227 |
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