Verification of the Potential Targets of the Herbal Prescription Sochehwan for Drug Repurposing Processes as Deduced by Network Pharmacology
| العنوان: | Verification of the Potential Targets of the Herbal Prescription Sochehwan for Drug Repurposing Processes as Deduced by Network Pharmacology |
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| المؤلفون: | Jai-Eun Kim, Won-Hwan Park, Da-Hoon Kim, Ga-Ram Yu, Dong-Woo Lim |
| المصدر: | Processes Volume 9 Issue 11 Processes, Vol 9, Iss 2034, p 2034 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Bioengineering, TP1-1185, Traditional Chinese medicine, Computational biology, GO enrichment analysis, network pharmacology, Chemical Engineering (miscellaneous), Medicine, STAT3, QD1-999, drug repurposing, biology, business.industry, Chemical technology, Process Chemistry and Technology, Sochehwan, Chemistry, key target validation, Drug repositioning, STAT protein, biology.protein, Phosphorylation, Signal transduction, business, Systems pharmacology |
| الوصف: | Network pharmacology (NP) is a useful, emerging means of understanding the complex pharmacological mechanisms of traditional herbal medicines. Sochehwan (SCH) is a candidate herbal prescription for drug repurposing as it has been suggested to have beneficial effects on metabolic syndrome. In this study, NP was adopted to complement the shortcomings of literature-based drug repurposing strategies in traditional herbal medicine. We conducted in vitro studies to confirm the effects of SCH on potential pharmacological targets identified by NP analysis. Herbal compounds and molecular targets of SCH were explored and screened from a traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) and an oriental medicine advanced searching integrated system (OASIS). Forty-seven key targets selected from a protein-protein interaction (PPI) network were analyzed with gene ontology (GO) term enrichment and KEGG pathway enrichment analysis to identify relevant categories. The tumor necrosis factor (TNF) and mitogen-activated protein kinase (MAPK) signaling pathways were presented as significant signaling pathways with lowest p-values by NP analysis, which were downregulated by SCH treatment. The signal transducer and activator of transcription 3 (STAT3) was identified as a core key target by NP analysis, and its phosphorylation ratio was confirmed to be significantly suppressed by SCH. In conclusion, the NP-based approach used for target prediction and experimental data obtained from Raw 264.7 cells strongly suggested that SCH can attenuate inflammatory status by modulating the phosphorylation status of STAT3. |
| وصف الملف: | application/pdf |
| تدمد: | 2227-9717 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2417a57d8d2f7a2d4146c43a59bc81f https://doi.org/10.3390/pr9112034 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e2417a57d8d2f7a2d4146c43a59bc81f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22279717 |
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