Expression of the genes for insecticidal crystal proteins inBacillus thuringiensis: cryIVA, notcryIVB, is transcribed by RNA polymerase containingÏHand that containingÏE
| العنوان: | Expression of the genes for insecticidal crystal proteins inBacillus thuringiensis: cryIVA, notcryIVB, is transcribed by RNA polymerase containingÏHand that containingÏE |
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| المؤلفون: | Tomoyuki Nishimoto, Tohru Komano, Kentaro Ihara, Hajime Yoshisue, Hiroshi Sakai |
| المصدر: | FEMS Microbiology Letters. 127:65-72 |
| بيانات النشر: | Oxford University Press (OUP), 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | DNA, Bacterial, Time Factors, Transcription, Genetic, Bacterial Toxins, Molecular Sequence Data, Bacillus thuringiensis, Sigma Factor, Bacillus subtilis, Biology, Regulon, Microbiology, Hemolysin Proteins, chemistry.chemical_compound, Bacterial Proteins, Species Specificity, Transcription (biology), Sigma factor, RNA polymerase, Gene expression, Genetics, Transcriptional regulation, Promoter Regions, Genetic, Molecular Biology, Spores, Bacterial, Bacillus thuringiensis Toxins, Base Sequence, fungi, Structural gene, DNA-Directed RNA Polymerases, Gene Expression Regulation, Bacterial, biology.organism_classification, Molecular biology, Endotoxins, chemistry, Genes, Bacterial, Mutation |
| الوصف: | To investigate the mechanism of transcriptional regulation of cryIVA and cryIVB, encoding 130-kDa dipteran-active crystal proteins, in Bacillus thuringiensis subsp. israelensis, we introduced each gene into several sporulation mutants of Bacillus subtilis. A spoIIG mutation, the wild-type gene of which encodes sigma E precursor, completely blocked the cryIVB transcription. In contrast, low but detectable transcription of cryIVA was observed in the spoIIG mutant. In the wild-type B. subtilis, no transcription of cryIVB was detected before T2 (2 h after the onset of stationary phase), while the cryIVA transcription started at the late exponential phase at low levels. Furthermore, in a wild-type strain of B. thuringiensis subsp. israelensis, transcription of cryIVA began earlier than that of genes encoding other crystal components, cryIVB and cytA. A consensus sequence recognized by an RNA polymerase containing sigma H of B. subtilis was found upstream of the transcription start point of cryIVA, which overlapped with that recognized by sigma E. |
| تدمد: | 1574-6968 0378-1097 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e326c2edfa51bd10f5bc63c0d2565af6 https://doi.org/10.1111/j.1574-6968.1995.tb07451.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e326c2edfa51bd10f5bc63c0d2565af6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15746968 03781097 |
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