Tumor‐derived exosomes influence the cell cycle and cell migration of human esophageal cancer cell lines
| العنوان: | Tumor‐derived exosomes influence the cell cycle and cell migration of human esophageal cancer cell lines |
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| المؤلفون: | Nobufumi Sekino, Soichiro Hirasawa, Takahiro Ryuzaki, Hisahiro Matsubara, Hiroshi Suito, Yasunori Matsumoto, Tadashi Shiraishi, Masahiko Takahashi, Toshiki Kamata, Takeshi Toyozumi, Kazuya Kinoshita, Kentaro Murakami, Masayuki Kano |
| المصدر: | Cancer Science |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Time Factors, Down-Regulation, Gene Expression, Biology, migration, Exosomes, Exosome, 03 medical and health sciences, 0302 clinical medicine, Cell, Molecular, and Stem Cell Biology, Cell Movement, Cell Line, Tumor, exosome, Humans, Extracellular structure organization, Cell Proliferation, Wound Healing, Cell growth, Cell Cycle, Optical Imaging, G1 Phase, Cell migration, Original Articles, General Medicine, Cell cycle, Actins, esophageal squamous cell carcinoma, Up-Regulation, Phenotype, 030104 developmental biology, fucci, Oncology, Cell culture, Tumor progression, 030220 oncology & carcinogenesis, Cancer cell, Disease Progression, Cancer research, Original Article |
| الوصف: | Our laboratory previously reported the usefulness as biomarkers of exosomes in the plasma of esophageal squamous cell carcinoma (ESCC) patients. However, the influence of tumor‐derived exosomes on the tumor itself and underlying mechanisms remain unclear. We here report changes in the phenotype and gene expression when cancer cells exist in an environment with tumor‐derived exosomes. The exosomes were isolated from the culture medium of human ESCC cells (TE2, T.Tn) by ultracentrifugation; cell proliferation assay, wound‐healing assay, and fluorescence imaging of the cell cycle were performed to clarify the phenotypic changes in the high concentration of tumor‐derived exosomes. Gene expression changes were also assessed by mRNA microarray, and the data were analyzed by gene set enrichment analysis (GSEA). The data revealed that the proliferation of both TE2 and T.Tn was inhibited, and cell migration ability was upregulated in the exosome exposure group (P The wound closure rate was compared between groups with or without cancer‐derived exosome exposure. There was a significant difference between 10 μg/mL exosome concentration and control at 12 and 24 h in both TE2 and T.Tn (P |
| تدمد: | 1349-7006 1347-9032 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3651b0a5f62e57551d5f76c704362c1 https://doi.org/10.1111/cas.14660 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e3651b0a5f62e57551d5f76c704362c1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13497006 13479032 |
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