Improvement Activity of 1-Deoxynojirimycin in the Growth of Dairy Goat Primary Mammary Epithelial Cell through Upregulating LEF-1 Expression
| العنوان: | Improvement Activity of 1-Deoxynojirimycin in the Growth of Dairy Goat Primary Mammary Epithelial Cell through Upregulating LEF-1 Expression |
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| المؤلفون: | Hongfu Zhang, Ming Liu, Shengyue Ji, Yuping Zhang |
| المصدر: | BioMed Research International BioMed Research International, Vol 2018 (2018) |
| بيانات النشر: | Hindawi Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, 1-Deoxynojirimycin, Article Subject, Lymphoid Enhancer-Binding Factor 1, Mammary gland, lcsh:Medicine, Biology, General Biochemistry, Genetics and Molecular Biology, Mice, 03 medical and health sciences, chemistry.chemical_compound, Mammary Glands, Animal, medicine, Animals, Breast, RNA, Messenger, Cell Proliferation, Gene knockdown, FGF10, General Immunology and Microbiology, Cell growth, Goats, lcsh:R, Epithelial Cells, General Medicine, Epithelium, In vitro, Up-Regulation, Cell biology, Wnt Proteins, 030104 developmental biology, medicine.anatomical_structure, chemistry, Female, Morus, Signal transduction, Research Article |
| الوصف: | LEF-1/wnt10b is one of the most important signaling pathways regulating mammary gland growth and development and is also a potential target for molecular breeding. In this work, 1-deoxynojirimycin (DNJ), a natural alkaloid extracted from plant mulberry or microorganism, was found to have a positive activity in primary breast epithelial cell growth of dairy goats. The findings showed that, compared to the control, 6 μM DNJ in the DMEM/F12 mediumin vitrogreatly improved the density of dairy goat breast epithelial cell and significantly increased the LEF-1 mRNA level (P<0.01) and thus enhanced cell growth. In addition, DNJ displayed a similar function in alleviating the growth suppression of epithelial cell and the decrease of LEF-1 mRNA level resulting from lentiviral-mediated LEF-1 knockdown. Simultaneously, no significant change of the mRNA levels of IGF-1 and Fgf10, the other two key regulators in mammary gland growth and development, could be detected. Furthermore, the mammary duct of DNJ-fed mouse illustrated a better development accompanied with a higher LET-1 mRNA level than that of the control. In conclusion, DNJ could improve breast epithelial cell growth through upregulating LEF-1 expression, which supplied a new means in studying mammary gland growth and development. |
| وصف الملف: | text/xhtml |
| تدمد: | 2314-6141 2314-6133 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3f0cf199dffbfc0e7b48582017fc51e https://doi.org/10.1155/2018/7809512 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e3f0cf199dffbfc0e7b48582017fc51e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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