Systemic Lipopolysaccharide-Induced Pain Sensitivity and Spinal Inflammation Were Reduced by Minocycline in Neonatal Rats
| العنوان: | Systemic Lipopolysaccharide-Induced Pain Sensitivity and Spinal Inflammation Were Reduced by Minocycline in Neonatal Rats |
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| المؤلفون: | Norma B. Ojeda, Lu-Tai Tien, Cheng-Ta Hsieh, Yih-Jing Lee, Hyun Joon Lee, Xiaoli Dai, Lir-Wan Fan |
| المصدر: | International Journal of Molecular Sciences, Vol 19, Iss 10, p 2947 (2018) International Journal of Molecular Sciences Volume 19 Issue 10 |
| بيانات النشر: | MDPI AG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Lipopolysaccharides, Male, 0301 basic medicine, Interleukin-1beta, Rats, Sprague-Dawley, lcsh:Chemistry, 0302 clinical medicine, minocycline, Prostaglandin E2, lcsh:QH301-705.5, Spectroscopy, Microglia, lipopolysaccharide, General Medicine, Minocycline, Anti-Bacterial Agents, 3. Good health, Computer Science Applications, medicine.anatomical_structure, Allodynia, Spinal Cord, Hyperalgesia, Female, medicine.symptom, Astrocyte, medicine.drug, medicine.medical_specialty, Inflammation, Dinoprostone, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Internal medicine, medicine, spinal cord inflammation, Animals, Physical and Theoretical Chemistry, Molecular Biology, hyperalgesia, allodynia, business.industry, Organic Chemistry, Spinal cord, Rats, 030104 developmental biology, Endocrinology, lcsh:Biology (General), lcsh:QD1-999, Cyclooxygenase 2, business, 030217 neurology & neurosurgery |
| الوصف: | In this study, we investigated the effects of minocycline, a putative suppressor of microglial activation, on systemic lipopolysaccharide (LPS)-induced spinal cord inflammation, allodynia, and hyperalgesia in neonatal rats. Intraperitoneal (i.p.) injection of LPS (2 mg/kg) or sterile saline was performed in postnatal day 5 (P5) rat pups and minocycline (45 mg/kg) or vehicle (phosphate buffer saline PBS) was administered (i.p.) 5 min after LPS injection. The von Frey filament and tail-flick tests were performed to determine mechanical allodynia (a painful sensation caused by innocuous stimuli, e.g., light touch) and thermal hyperalgesia (a condition of altered perception of temperature), respectively, and spinal cord inflammation was examined 24 h after the administration of drugs. Systemic LPS administration resulted in a reduction of tactile threshold in the von Frey filament tests and pain response latency in the tail-flick test of neonatal rats. The levels of microglia and astrocyte activation, pro-inflammatory cytokine interleukin-1&beta (IL-1&beta ), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) in the spinal cord of neonatal rats were increased 24 h after the administration of LPS. Treatment with minocycline significantly attenuated LPS-induced allodynia, hyperalgesia, the increase in spinal cord microglia, and astrocyte activation, and elevated levels of IL-1&beta COX-2, and PGE2 in neonatal rats. These results suggest that minocycline provides protection against neonatal systemic LPS exposure-induced enhanced pain sensitivity (allodynia and hyperalgesia), and that the protective effects may be associated with its ability to attenuate LPS-induced microglia activation, and the levels of IL-1&beta COX-2, and PGE2 in the spinal cord of neonatal rats. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e422459ee136e41b094d1b053359b377 http://www.mdpi.com/1422-0067/19/10/2947 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e422459ee136e41b094d1b053359b377 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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