Staphylococcus simulans recombinant lysostaphin: Production, purification, and determination of antistaphylococcal activity
| العنوان: | Staphylococcus simulans recombinant lysostaphin: Production, purification, and determination of antistaphylococcal activity |
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| المؤلفون: | N V Lavrova, Z. M. Galushkina, R S Ovchinnikov, Shaginian Ia, A V Demidenko, A. V. Grishin, M Iu Chernukha, Anna S. Karyagina, I. S. Boksha, Vladimir G. Lunin, L. R. Avetisian, A.M. Umyarov, A. M. Lyashchuk |
| المصدر: | Biochemistry (Moscow). 81:502-510 |
| بيانات النشر: | Pleiades Publishing Ltd, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Staphylococcus aureus, Staphylococcus, 030106 microbiology, Peptidoglycan, medicine.disease_cause, Biochemistry, Microbiology, law.invention, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Disk Diffusion Antimicrobial Tests, law, Staphylococcus simulans, Escherichia coli, medicine, Biomass, Cloning, Molecular, biology, Lysostaphin, Temperature, General Medicine, biology.organism_classification, Staphylococcus haemolyticus, Recombinant Proteins, Anti-Bacterial Agents, chemistry, Recombinant DNA, Electrophoresis, Polyacrylamide Gel |
| الوصف: | Staphylococcus simulans lysostaphin is an endopeptidase lysing staphylococcus cell walls by cleaving pentaglycine cross-bridges in their peptidoglycan. A synthetic gene encoding S. simulans lysostaphin was cloned in Escherichia coli cells, and producer strains were designed. The level of produced biologically active lysostaphin comprised 6-30% of total E. coli cell protein (depending on E. coli M15 or BL21 producer) under batch cultivation conditions. New methods were developed for purification of lysostaphin without affinity domains and for testing its enzymatic activity. As judged by PAGE, the purified recombinant lysostaphin is of >97% purity. The produced lysostaphin lysed cells of Staphylococcus aureus and Staphylococcus haemolyticus clinical isolates. In vitro activity and general biochemical properties of purified recombinant lysostaphin produced by M15 or BL21 E. coli strains were identical to those of recombinant lysostaphin supplied by Sigma-Aldrich (USA) and used as reference in other known studies. The prepared recombinant lysostaphin represents a potential product for development of enzymatic preparation for medicine and veterinary due to the simple purification scheme enabling production of the enzyme of high purity and antistaphylococcal activity. |
| تدمد: | 1608-3040 0006-2979 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e46983115d6c2ef3c2fc2ee9a189a62f https://doi.org/10.1134/s0006297916050072 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....e46983115d6c2ef3c2fc2ee9a189a62f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16083040 00062979 |
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