Treatment Efficiency of Different Routes of Bone Marrow-Derived Mesenchymal Stem Cell Injection in Rat Liver Fibrosis Model
| العنوان: | Treatment Efficiency of Different Routes of Bone Marrow-Derived Mesenchymal Stem Cell Injection in Rat Liver Fibrosis Model |
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| المؤلفون: | Amany Osama, Naglaa K. Idriss, Dina Sabry, Hayam G. Sayyed |
| المصدر: | Cellular Physiology and Biochemistry, Vol 48, Iss 5, Pp 2161-2171 (2018) |
| بيانات النشر: | Cell Physiol Biochem Press GmbH & Co KG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Liver Cirrhosis, Male, 0301 basic medicine, Pathology, medicine.medical_specialty, Physiology, medicine.medical_treatment, Liver fibrosis, Connective tissue, Bone Marrow Cells, Mesenchymal Stem Cell Transplantation, lcsh:Physiology, Transforming Growth Factor beta1, lcsh:Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, Fibrosis, medicine, Animals, lcsh:QD415-436, Carbon Tetrachloride, Transplantation route, Keratin-18, lcsh:QP1-981, Hepatocyte Growth Factor, business.industry, Growth factor, Mesenchymal stem cell, Connective Tissue Growth Factor, Mesenchymal Stem Cells, medicine.disease, Actins, Rats, Vascular endothelial growth factor, Transplantation, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Liver, chemistry, Administration, Intravenous, Liver function, Bone marrow, business, Spleen, BM-MSCs |
| الوصف: | Background/Aims: The most appropriate route for bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation in the management of liver fibrosis remains controversial. This study investigated the therapeutic efficacy of intravenous and intrasplenic BM-MSC transplantation on carbon tetrachloride (CCl4)-induced rat liver fibrosis. Methods: Fifty rats were divided into 5 groups (n = 10 rats per group): healthy control group, CCl4 group, CCl4/ recovery group, CCl4/BM-MSC intravenous group, and CCl4/BM-MSC intrasplenic group. BM-MSCs were isolated, labeled with green fluorescent protein (GFP), and injected into fibrotic rats either intravenously or intrasplenically. Gene expression of interleukins (IL-1β and IL-6), interferon (INF)-γ, hepatic growth factor, and the hepatocyte-specific marker cytokeratin 18 was estimated by quantitative real-time reverse transcription-polymerase chain reaction. Vascular endothelial growth factor and connective tissue growth factor was detected by western blot analysis and enzyme-linked immunosorbent assay, respectively. At 2 weeks after intravenous and intrasplenic BM-MSC injections, GFP-positive cells were detected in liver tissue. Results: Both routes achieved a similar enhancement of liver function, which was confirmed by histopathological examination. The intravenous route was more effective than the intrasplenic route in reducing gene expression levels of IL-1β, IL-6, and INF-γ. However, fibrotic changes were still observed in the recovery group. Conclusion: Intravenous BM-MSC injection was an efficient and appropriate route for BM-MSC transplantation for the management of liver fibrosis. |
| اللغة: | English |
| تدمد: | 1421-9778 1015-8987 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e499f5ef50355b20363989802076c240 https://www.karger.com/Article/FullText/492558 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e499f5ef50355b20363989802076c240 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219778 10158987 |
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