Differential Cytokine Production and Toll-Like Receptor Signaling Pathways by Candida albicans Blastoconidia and Hyphae
| العنوان: | Differential Cytokine Production and Toll-Like Receptor Signaling Pathways by Candida albicans Blastoconidia and Hyphae |
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| المؤلفون: | Bart Jan Kullberg, Ineke Verschueren, Chantal A. A. van der Graaf, Mihai G. Netea, Jos W. M. van der Meer |
| المصدر: | Infection and Immunity, 73, 11, pp. 7458-64 Infection and Immunity, 73, 7458-64 |
| بيانات النشر: | American Society for Microbiology, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Time Factors, Hypha, medicine.medical_treatment, Immunology, Phenotypic switching, Hyphae, Biology, Microbiology, Blastoconidium, Proinflammatory cytokine, Invasive mycoses and compromised host [N4i 2], Mice, Candida albicans, Effective Primary Care and Public Health [EBP 3], medicine, Animals, Humans, Mice, Knockout, Toll-like receptor, fungi, Toll-Like Receptors, Candidiasis, Spores, Fungal, biology.organism_classification, Toll-Like Receptor 2, Corpus albicans, Mice, Inbred C57BL, Toll-Like Receptor 4, Pathogenesis and modulation of inflammation [N4i 1], Infectious Diseases, Cytokine, Gene Expression Regulation, Leukocytes, Mononuclear, Cytokines, Parasitology, Microbial pathogenesis and host defense [UMCN 4.1], Fungal and Parasitic Infections, Infection and autoimmunity [NCMLS 1], Spleen, Signal Transduction |
| الوصف: | Toll-like receptors (TLR) are crucial for an efficient antifungal defense. We investigated the differential recognition of blastoconidia and hyphae of Candida albicans by TLRs. In contrast to Candida blastoconidia, which stimulated large amounts of gamma interferon (IFN-γ), the tissue-invasive Candida hyphae did not stimulate any IFN-γ by human peripheral blood mononuclear cells (PBMC) or murine splenic lymphocytes. After stimulation with blastoconidia, the production of IFN-γ was TLR4 dependent, as shown by the significantly decreased IFN-γ production in anti-TLR4-treated PBMC and in splenic lymphocytes from TLR4-defective ScCr mice. In addition, peritoneal macrophages from ScCr mice produced less tumor necrosis factor α (TNF-α) than macrophages of control mice did when stimulated with Candida blastoconidia, but not with hyphae, indicating that TLR4-mediated signals are lost during hyphal germination. In contrast, macrophages from TLR2 knockout mice had a decreased production of TNF-α in response to both Candida blastoconidia and hyphae. Candida hyphae stimulated production of interleukin-10 through TLR2-dependent mechanisms. In conclusion, TLR4 mediates proinflammatory cytokine induction after Candida stimulation, whereas Candida recognition by TLR2 leads mainly to anti-inflammatory cytokine release. TLR4-mediated proinflammatory signals are lost during germination of Candida blastoconidia into hyphae. Phenotypic switching during germination may be an important escape mechanism of C. albicans , resulting in counteracting host defense. |
| وصف الملف: | application/pdf |
| تدمد: | 1098-5522 0019-9567 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e509320cac7123166960a26aceeec97d https://doi.org/10.1128/iai.73.11.7458-7464.2005 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e509320cac7123166960a26aceeec97d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
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