Coated and Hollow Microneedle-Mediated Intradermal Immunization in Mice with Diphtheria Toxoid Loaded Mesoporous Silica Nanoparticles
| العنوان: | Coated and Hollow Microneedle-Mediated Intradermal Immunization in Mice with Diphtheria Toxoid Loaded Mesoporous Silica Nanoparticles |
|---|---|
| المؤلفون: | Laura Woythe, Mara Leone, Gideon F.A. Kersten, Alexander Kros, Guangsheng Du, Koen van der Maaden, Joke A. Bouwstra, Wim Jiskoot, Stefan Romeijn |
| المصدر: | Pharmaceutical Research, 35(10), 189. American Association of Pharmaceutical Scientists Pharmaceutical Research Pharmaceutical Research, 35(10), 189 BASE-Bielefeld Academic Search Engine |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, animal structures, Materials science, Injections, Intradermal, Diphtheria Toxoid, Surface Properties, Drug Compounding, intradermal vaccination, Pharmaceutical Science, Nanoparticle, hollow microneedles, 02 engineering and technology, engineering.material, Chitosan, Mice, 03 medical and health sciences, chemistry.chemical_compound, Drug Delivery Systems, Immunogenicity, Vaccine, Coating, Zeta potential, Animals, Humans, Pharmacology (medical), Particle Size, mesoporous silica nanoparticles, Lipid bilayer, Pharmacology, Diphtheria toxin, Mice, Inbred BALB C, Immunogenicity, Organic Chemistry, Mesoporous silica, Silicon Dioxide, 021001 nanoscience & nanotechnology, 030104 developmental biology, Chemical engineering, chemistry, engineering, Nanoparticles, Molecular Medicine, Female, Immunization, 0210 nano-technology, Porosity, coated microneedles, Research Paper, Biotechnology |
| الوصف: | To examine the immunogenicity of diphtheria toxoid (DT) loaded mesoporous silica nanoparticles (MSNs) after coated and hollow microneedle-mediated intradermal immunization in mice. DT was loaded into MSNs and the nanoparticle surface was coated with a lipid bilayer (LB-MSN-DT). To prepare coated microneedles, alternating layers of negatively charged LB-MSN-DT and positively charged N-trimethyl chitosan (TMC) were coated onto pH-sensitive microneedle arrays via a layer-by-layer approach. Microneedle arrays coated with 5 or 3 layers of LB-MSN-DT were used to immunize mice and the elicited antibody responses were compared with those induced by hollow microneedle-injected liquid formulation of LB-MSN-DT. Liquid DT formulation with and without TMC (DT/TMC) injected by a hollow microneedle were used as controls. LB-MSN-DT had an average size of about 670 nm and a zeta potential of -35 mV. The encapsulation efficiency of DT in the nanoparticles was 77%. The amount of nano-encapsulated DT coated onto the microneedle array increased linearly with increasing number of the coating layers. Nano-encapsulated DT induced stronger immune responses than DT solution when delivered intradermally via hollow microneedles, but not when delivered via coated microneedles. Both the nano-encapsulation of DT and the type of microneedles affect the immunogenicity of the antigen. PURPOSE METHODS RESULTS CONCLUSION |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e522c86a21e60d10ce8f609c90ef1777 https://hdl.handle.net/1887/64967 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e522c86a21e60d10ce8f609c90ef1777 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |