Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells
| العنوان: | Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells |
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| المؤلفون: | Koji Hayakawa, Nobuyuki Fujiwara, Nao Kitamura, Koichi Sato, Takashi Ohama |
| المصدر: | The Journal of Biochemistry. 170:131-138 |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | biology, Neurite, Chemistry, Neuronal Outgrowth, Mechanistic Target of Rapamycin Complex 2, General Medicine, CREB, Biochemistry, mTORC2, N2a cell, Cell biology, Mice, Phosphoprotein Phosphatases, biology.protein, Neuron differentiation, Animals, Humans, Phosphorylation, Molecular Biology, Protein kinase B, Cells, Cultured, PI3K/AKT/mTOR pathway |
| الوصف: | Understanding the molecular mechanism of neuronal differentiation is important to overcome the incurable diseases caused by nervous system damage. Neurite outgrowth is prerequisite for neuronal differentiation and regeneration, and cAMP response element-binding protein (CREB) is one of the major transcriptional factors positively regulating this process. Neuronal differentiation stimuli activate mammalian target of rapamycin (mTOR) complex 2 (mTORC2)/Akt signalling to phosphorylate CREB; however, the precise molecular mechanism of this event has not been fully understood. In this manuscript, we show that neuronal differentiation stimuli increased a protein level of protein phosphatase 6 (PP6), a member of type 2A Ser/Thr protein phosphatases. PP6 knockdown suppressed mTORC2/Akt/CREB signalling and results in failure of neurite outgrowth. SIN1 is a unique component of mTORC2 that enhances mTORC2 activity towards Akt when it is in dephosphorylated form. We found PP6 knockdown increased SIN1 phosphorylation. These data suggest that PP6 may positively regulate neurite outgrowth by dephosphorylating SIN1 to activate mTORC2/Akt/CREB signalling. |
| تدمد: | 1756-2651 0021-924X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5b67dcd0b69e5ded8779af2781403e5 https://doi.org/10.1093/jb/mvab028 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e5b67dcd0b69e5ded8779af2781403e5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17562651 0021924X |
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