Cell sorting actuated by a microfluidic inertial vortex
| العنوان: | Cell sorting actuated by a microfluidic inertial vortex |
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| المؤلفون: | James N. Fullerton, Andrew Want, Robyn H. Pritchard, Edward Banham-Hall, Richard D. Gold, Fred Hussain, Mette Funding la Cour, Mikhail E Bashtanov, Alexander A Zhukov, Anthony Hailes, Salman Samson Rogers |
| المصدر: | Lab on a Chip. 19:2456-2465 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Materials science, Cell Survival, Microfluidics, Biomedical Engineering, Bioengineering, Cell Separation, 02 engineering and technology, 01 natural sciences, Biochemistry, Multiplexing, Jurkat Cells, Lab-On-A-Chip Devices, Miniaturization, Humans, sort, Computer Simulation, 010401 analytical chemistry, Sorting, General Chemistry, Cell sorting, 021001 nanoscience & nanotechnology, Microspheres, 0104 chemical sciences, Vortex, Leukocytes, Mononuclear, 0210 nano-technology, Biological system, Immortalised cell line |
| الوصف: | The sorting of specific cell populations is an established tool in biological research, with new applications demanding greater cell throughput, sterility and elimination of cross-contamination. Here we report 'vortex-actuated cell sorting' (VACS), a new technique that deflects cells individually, via the generation of a transient microfluidic vortex by a thermal vapour bubble: a novel mechanism, which is able to sort cells based on fluorescently-labelled molecular markers. Using in silico simulation and experiments on beads, an immortal cell line and human peripheral blood mononuclear cells (PBMCs), we demonstrate high-purity and high-recovery sorting with input rates up to 104 cells per s and switching speeds comparable to existing techniques (>40 kHz). A tiny footprint (1 × 0.25 mm) affords miniaturization and the potential to achieve multiplexing: a crucial step in increasing processing rate. Simple construction using biocompatible materials potentially minimizes cost of fabrication and permits single-use sterile cartridges. We believe VACS potentially enables parallel sorting at throughputs relevant to cell therapy, liquid biopsy and phenotypic screening. |
| تدمد: | 1473-0189 1473-0197 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5fa2a6ef6bc184cdcf33c0e3e2633ad https://doi.org/10.1039/c9lc00120d |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e5fa2a6ef6bc184cdcf33c0e3e2633ad |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14730189 14730197 |
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