التفاصيل البيبلوغرافية
العنوان:
The human liver lipidome is significantly related to the lipid composition and aggregation susceptibility of low-density lipoprotein (LDL) particles
المؤلفون:
Mari Lahelma , Sami Qadri , Noora Ahlholm , Kimmo Porthan , Maija Ruuth , Anne Juuti , Matej Orešič , Tuulia Hyötyläinen , Katariina Öörni , Hannele Yki-Järvinen
المساهمون:
Department of Medicine, HUS Internal Medicine and Rehabilitation, Clinicum, Medicum, HUS Abdominal Center, Molecular and Integrative Biosciences Research Programme, Biosciences, Hannele Yki-Järvinen Research Group
سنة النشر:
2022
مصطلحات موضوعية:
Adult , Sfingolipidit , Lihavuus , keramidit , ei-alkoholiperäinen rasvamaksatauti , Lipoproteins , LDL , NAFLD , rasvamaksatauti , Humans , lipidomiikka , Obesity , Triglycerides , liver biopsy , fatty liver , Sphingolipids , ceramides , Liver Diseases , Ateroskleroosi , rasvamaksa , maksabiopsia , aggregation , aggregaatio , non-alcoholic fatty liver disease , Middle Aged , Lipoproteiinit , Atherosclerosis , Sphingomyelins , Lipoproteins, LDL , Liver , 3121 General medicine, internal medicine and other clinical medicine , fatty liver disease , lipidomics , Cardiology and Cardiovascular Medicine , Maksasairaudet
الوصف:
Publisher Copyright: © 2022 The Authors Background and aims: The susceptibility of low-density lipoprotein (LDL) to aggregation predicts atherosclerotic cardiovascular disease. However, causes of interindividual variation in LDL lipid composition and aggregation susceptibility remain unclear. We examined whether the lipid composition and aggregation susceptibility of LDL reflect the lipid composition of the human liver. Methods: Liver biopsies and blood samples for isolation of LDL particles were obtained from 40 obese subjects (BMI 45.9 ± 6.1 kg/m2, age 43 ± 8 years). LDL was isolated using sequential ultracentrifugation and lipidomic analyses of liver and LDL samples were determined using ultra-high performance liquid chromatography–mass spectrometry. LDL aggregation susceptibility ex vivo was analyzed by inducing aggregation by human recombinant secretory sphingomyelinase and following aggregate formation. Results: The composition (acyl carbon number and double bond count) of hepatic triglycerides, phosphatidylcholines, and sphingomyelins (SMs) was closely associated with that of LDL particles. Hepatic dihydroceramides and ceramides were positively correlated with concentrations of the corresponding SM species in LDL as well with LDL aggregation. These relationships remained statistically significant after adjustment for age, sex, and body mass index. Conclusions: Lipid composition of LDL reflects that of the human liver in obese patients. Changes in hepatic sphingolipid metabolism may contribute to interindividual variation of LDL lipid composition and susceptibility to aggregation.
اللغة:
English
URL الوصول:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e61a9fc94a7971e46ed046b96fc45d5e http://hdl.handle.net/10138/353504
حقوق:
OPEN
رقم الأكسشن:
edsair.doi.dedup.....e61a9fc94a7971e46ed046b96fc45d5e
قاعدة البيانات:
OpenAIRE
