Getting the Most Out of Your Crystals: Data Collection at the New High-Flux, Microfocus MX Beamlines at NSLS-II
| العنوان: | Getting the Most Out of Your Crystals: Data Collection at the New High-Flux, Microfocus MX Beamlines at NSLS-II |
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| المؤلفون: | Wuxian Shi, Sandra B. Gabelli, Nam Chu, S. Maheshwari, Philip A. Cole, Michelle S. Miller, L. Mario Amzel, Martin Fuchs, Jean Jakoncic, Yuan Gao, Alexei S. Soares |
| المصدر: | Molecules Volume 24 Issue 3 Molecules, Vol 24, Iss 3, p 496 (2019) |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Models, Molecular, Protein Conformation, Computer science, 030303 biophysics, PHM, Pharmaceutical Science, Crystallographic data, Crystallography, X-Ray, Article, Analytical Chemistry, Computational science, law.invention, lcsh:QD241-441, Crystal, Phosphatidylinositol 3-Kinases, 03 medical and health sciences, lcsh:Organic chemistry, law, Drug Discovery, nonhomogeneous crystals, Pyrophosphatases, Physical and Theoretical Chemistry, 030304 developmental biology, 0303 health sciences, Data collection, multicrystal, PI3Kα, Akt1, business.industry, Organic Chemistry, Proteins, Automation, CDP-Chase, Synchrotron, High flux, microdiffraction, Chemistry (miscellaneous), microfocus, NSLS-II, Trajectory, vector data collection, Molecular Medicine, business, Volume (compression) |
| الوصف: | Advances in synchrotron technology are changing the landscape of macromolecular crystallography. The two recently opened beamlines at NSLS-II&mdash AMX and FMX&mdash deliver high-flux microfocus beams that open new possibilities for crystallographic data collection. They are equipped with state-of-the-art experimental stations and automation to allow data collection on previously intractable crystals. Optimized data collection strategies allow users to tailor crystal positioning to optimally distribute the X-ray dose over its volume. Vector data collection allows the user to define a linear trajectory along a well diffracting volume of the crystal and perform rotational data collection while moving along the vector. This is particularly well suited to long, thin crystals. We describe vector data collection of three proteins&mdash Akt1, PI3K&alpha and CDP-Chase&mdash to demonstrate its application and utility. For smaller crystals, we describe two methods for multicrystal data collection in a single loop, either manually selecting multiple centers (using H108A-PHM as an example), or &ldquo raster-collect&rdquo a more automated approach for a larger number of crystals (using CDP-Chase as an example). |
| وصف الملف: | application/pdf |
| تدمد: | 1420-3049 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6e8f669b0b63c9247b15bcbe045ecb8 https://doi.org/10.3390/molecules24030496 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e6e8f669b0b63c9247b15bcbe045ecb8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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