Combinatorial screening of biochemical and physical signals for phenotypic regulation of stem cell-based cartilage tissue engineering
| العنوان: | Combinatorial screening of biochemical and physical signals for phenotypic regulation of stem cell-based cartilage tissue engineering |
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| المؤلفون: | Ming Kong, Wujin Sun, KangJu Lee, Amr A. Abdeen, Su Ryon Shin, Somali Chaterji, Yu Bin Lee, Han-Jun Kim, Ha Neul Lee, Sang Jin Lee, Praveen Bandaru, Oju Jeon, Jung-Youn Shin, Daniel S. Alt, Ali Khademhosseini, Eben Alsberg, Junmin Lee |
| المصدر: | Science Advances |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Cartilage, Articular, Materials Science, Chondrocyte hypertrophy, Biocompatible Materials, 02 engineering and technology, Mechanotransduction, Cellular, 03 medical and health sciences, medicine, Health and Medicine, Mechanotransduction, Research Articles, 030304 developmental biology, 0303 health sciences, Multidisciplinary, Tissue Engineering, Chemistry, Cartilage, Stem Cells, Mesenchymal stem cell, Wnt signaling pathway, Biomaterial, SciAdv r-articles, Cell Differentiation, Mesenchymal Stem Cells, 021001 nanoscience & nanotechnology, Chondrogenesis, Cell biology, medicine.anatomical_structure, Phenotype, Stem cell, 0210 nano-technology, Research Article |
| الوصف: | Multicomponent biomaterials using a high-throughput system regulate hyaline or hypertrophic chondrogenesis of hMSCs. Despite great progress in biomaterial design strategies for replacing damaged articular cartilage, prevention of stem cell-derived chondrocyte hypertrophy and resulting inferior tissue formation is still a critical challenge. Here, by using engineered biomaterials and a high-throughput system for screening of combinatorial cues in cartilage microenvironments, we demonstrate that biomaterial cross-linking density that regulates matrix degradation and stiffness—together with defined presentation of growth factors, mechanical stimulation, and arginine-glycine-aspartic acid (RGD) peptides—can guide human mesenchymal stem cell (hMSC) differentiation into articular or hypertrophic cartilage phenotypes. Faster-degrading, soft matrices promoted articular cartilage tissue formation of hMSCs by inducing their proliferation and maturation, while slower-degrading, stiff matrices promoted cells to differentiate into hypertrophic chondrocytes through Yes-associated protein (YAP)–dependent mechanotransduction. in vitro and in vivo chondrogenesis studies also suggest that down-regulation of the Wingless and INT-1 (WNT) signaling pathway is required for better quality articular cartilage-like tissue production. |
| تدمد: | 2375-2548 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6fa8639d0b1e04e044418ef7e716a00 https://pubmed.ncbi.nlm.nih.gov/32494742 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e6fa8639d0b1e04e044418ef7e716a00 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23752548 |
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