Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells
| العنوان: | Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells |
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| المؤلفون: | Hinco J. Gierman, Rogier Versteeg, Pavel Matula, Stanislav Kozubek, Eva Bártová, Gabriela Šustáčková, Darya Y. Orlova, Andrei V. Chernyshev, Roman Stoklasa, Ruslan N. Medvedev, Soňa Legartová, Lenka Stixová |
| المساهمون: | Other departments, Cancer Center Amsterdam, Oncogenomics |
| المصدر: | Journal of cellular biochemistry, 113(11), 3313-3329. Wiley-Liss Inc. Journal of Cellular Biochemistry |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Cell division, Chromosomal Proteins, Non-Histone, Mitosis, Coiled Bodies, Hydroxamic Acids, Biochemistry, Cell Line, Chromatin bridge, Histones, 03 medical and health sciences, Mice, 0302 clinical medicine, Prophase, Heterochromatin, Animals, Humans, RNA, Messenger, Molecular Biology, Interphase, 030304 developmental biology, Fluorescent Dyes, Protein Synthesis Inhibitors, 0303 health sciences, biology, Cell Biology, Photochemical Processes, Chromatin, Cell biology, Histone Deacetylase Inhibitors, Histone, Microscopy, Fluorescence, Chromobox Protein Homolog 5, Premature chromosome condensation, biology.protein, Dactinomycin, 030217 neurology & neurosurgery, Cell Nucleolus |
| الوصف: | Although it is well known that chromosomes are non-randomly organized during interphase, it is not completely clear whether higher-order chromatin structure is transmitted from mother to daughter cells. Therefore, we addressed the question of how chromatin is rearranged during interphase and whether heterochromatin pattern is transmitted after mitosis. We additionally tested the similarity of chromatin arrangement in sister interphase nuclei. We noticed a very active cell rotation during interphase, especially when histone hyperacetylation was induced or transcription was inhibited. This natural phenomenon can influence the analysis of nuclear arrangement. Using photoconversion of Dendra2-tagged core histone H4 we showed that the distribution of chromatin in daughter interphase nuclei differed from that in mother cells. Similarly, the nuclear distribution of heterochromatin protein 1 beta (HP1 beta) was not completely identical in mother and daughter cells. However, identity between mother and daughter cells was in many cases evidenced by nucleolar composition. Moreover, morphology of nucleoli, HP1 beta protein, Cajal bodies, chromosome territories, and gene transcripts were identical in sister cell nuclei. We conclude that the arrangement of interphase chromatin is not transmitted through mitosis, but the nuclear pattern is identical in naturally synchronized sister cells. It is also necessary to take into account the possibility that cell rotation and the degree of chromatin condensation during functionally specific cell cycle phases might influence our view of nuclear architecture. J. Cell. Biochem. 113: 33133329, 2012. (C) 2012 Wiley Periodicals, Inc |
| تدمد: | 1097-4644 0730-2312 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7091a5a2044fe933096532ba599f708 https://pubmed.ncbi.nlm.nih.gov/22644811 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....e7091a5a2044fe933096532ba599f708 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974644 07302312 |
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