Additional file 3: Figure S1. The establishment of transgenic ice plant callus expressing the yellow fluorescent protein (YFP). (A) Representative untransformed ice plant callus grown on CIM with (1) 0 mg L−1 and (2) 25 mg L−1 glufosinate ammonium. Bar = 1 cm. (A-3) Fresh weight of untransformed ice plant callus grown on CIM with 0, 25, 50, 75, and 100 mg L−1 glufosinate ammonium (GLA). ΔFW: increased fresh weight mass. Average values of the increased fresh weight from at least three independent experiments. *P